Publikationsdatenbank

Genome-wide association reveals host-specific genomic traits in Escherichia coli (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Tiwari, Sumeet K.

van der Putten, Boas C. L.

Fuchs, Thilo M.

Vinh, Trung N.

Bootsma, Martin

Oldenkamp, Rik

La Ragione, Roberto

Matamoros, Sebastien

Hoa, Ngo T.

Berens, Christian

Leng, Joy

Álvarez, Julio

Ferrandis-Vila, Marta

Ritchie, Jenny M.

Fruth, Angelika

Schwarz, Stefan (WE 7)

Domínguez, Lucas

Ugarte-Ruiz, María

Bethe, Astrid (WE 7)

Huber, Charlotte (WE 7)

Johanns, Vanessa

Stamm, Ivonne

Wieler, Lothar H. (WE 7)

Ewers, Christa

Fivian-Hughes, Amanda

Schmidt, Herbert

Menge, Christian

Semmler, Torsten

Schultsz, Constance

Quelle

BMC biology

Bandzählung: 21

Heftzählung: 1

Seiten: Artikel 76

ISSN: 1741-7007

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/37038177/

DOI: 10.1186/s12915-023-01562-w

Pubmed: 37038177

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Escherichia coli is an opportunistic pathogen which colonizes various host species. However, to what extent genetic lineages of E. coli are adapted or restricted to specific hosts and the genomic determinants of such adaptation or restriction is poorly understood.

Results:
We randomly sampled E. coli isolates from four countries (Germany, UK, Spain, and Vietnam), obtained from five host species (human, pig, cattle, chicken, and wild boar) over 16 years, from both healthy and diseased hosts, to construct a collection of 1198 whole-genome sequenced E. coli isolates. We identified associations between specific E. coli lineages and the host from which they were isolated. A genome-wide association study (GWAS) identified several E. coli genes that were associated with human, cattle, or chicken hosts, whereas no genes associated with the pig host could be found. In silico characterization of nine contiguous genes (collectively designated as nan-9) associated with the human host indicated that these genes are involved in the metabolism of sialic acids (Sia). In contrast, the previously described sialic acid regulon known as sialoregulon (i.e. nanRATEK-yhcH, nanXY, and nanCMS) was not associated with any host species. In vitro growth experiments with a Δnan-9 E. coli mutant strain, using the sialic acids 5-N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) as sole carbon source, showed impaired growth behaviour compared to the wild-type.

Conclusion:
This study provides an extensive analysis of genetic determinants which may contribute to host specificity in E. coli. Our findings should inform risk analysis and epidemiological monitoring of (antimicrobial resistant) E. coli.