Publikationsdatenbank

Re: "Investigation of Macrolide resistance genotypes of Pasteurella multocida isolates from cattle and small ruminants" (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schink, Anne-Kathrin (WE 7)

Hanke, Dennis (WE 7)

Kostova, Valeria (WE 7)

Schwarz, Stefan (WE 7)

Quelle

Microbial drug resistance : MDR ; mechanisms, epidemiology, and disease

Bandzählung: 29

Heftzählung: 4

Seiten: 163 – 164

ISSN: 1076-6294

Sprache

Englisch

Verweise

URL (Volltext): https://pubmed.ncbi.nlm.nih.gov/36637809/

DOI: 10.1089/mdr.2022.0294

Pubmed: 36637809

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Macrolides are commonly used to control respiratory tract infections in ruminants, but the susceptibility of Pasteurella multocida strains has shown a decrease to macrolide antibiotics in the last decade. In this work we assessed the prevalence of macrolide resistance of 100 P. multocida isolates from ruminant hosts and studied the resistance genotypes with newly designed PCRs. Susceptibility to erythromycin and tilmicosin was tested using minimal inhibitory concentration (MIC) test strips. A newly designed PCR was used for the detection of macAB genes, and a PCR plus restriction enzyme-based technique was developed for detecting a 23S rRNA gene mutation at position 2059. Five bovine isolates with notably increased MICs (≥256 μg/mL for erythromycin and ≥32 μg/mL for tilmicosin) carried resistance genes msr(E) and mph(E) or the A2059G point mutation in the 23S rRNA gene. Over 73% strains from small ruminants and all bovine isolates were MacAB PCR positive. Bovine strains were less sensitive to macrolide antibiotics than isolates from small ruminants, and an increase in the prevalence of macrolide resistance in bovine P. multocida isolates has also been observed over time.