Publikationsdatenbank

The antigen recognition portion of African buffalo class I MHC is highly polymorphic, consistent with a complex pathogen challenge environment, and the 3' region suggests distinct haplotype configurations (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Obara, Isaiah (WE 13)

Nijhof, Ard (WE 13)

Atimnedi, Patrick

Mijele, Domnic

Nanteza, Anne

Elati, Khawla (WE 13)

Bishop, Richard

Quelle

Immunogenetics

Bandzählung: 75

Heftzählung: 2

Seiten: 115 – 132

ISSN: 0093-7711

Sprache

Englisch

Verweise

URL (Volltext): https://link.springer.com/article/10.1007/s00251-022-01287-0

DOI: 10.1007/s00251-022-01287-0

Pubmed: 36512055

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

African buffalo (Syncerus caffer) have been distinct from the Auroch lineage leading to domestic cattle for 5 million years, and are reservoirs of multiple pathogens, that affect introduced domestic cattle. To date, there has been no analysis of the class I MHC locus in African buffalo. We present the first data on African buffalo class I MHC, which demonstrates that gene and predicted protein coding sequences are approximately 86-87% similar to that of African domestic cattle in the peptide binding region. The study also shows concordance in the distribution of codons with elevated posterior probabilities of positive selection in the buffalo class I MHC and known antigen binding sites in cattle. Overall, the diversity in buffalo class I sequences appears greater than that in cattle, perhaps related to a more complex pathogen challenge environment in Africa. However, application of NetMHCpan suggested broad clustering of peptide binding specificities between buffalo and cattle. Furthermore, in the case of at least 20 alleles, critical peptide-binding residues appear to be conserved with those of cattle, including at secondary anchor residues. Alleles with six different length transmembrane regions were detected. This preliminary analysis suggests that like cattle, but unlike most other mammals, African buffalo appears to exhibit configuration (haplotype) variation in which the loci are expressed in distinct combinations.