Publikationsdatenbank

Vanin-1 is expressed in the airway epithelium of horses and has a higher abundance in the mucus of horses with severe equine asthma compared with healthy horses (2024)

Art

Poster

Autoren

Landmann, K. (WE 12)

Bartenschlager, F. (WE 12)

Kuropka, B.

Weise, C.

Gehlen, H. (WE 17)

Zeyner, A.

Gruber, A. D. (WE 12)

Schnabel, C. L.

Mundhenk, L. (WE 12)

Kongress

European Congress of Veterinary Pathology and Clinical Pathology (ESVP/ECVP/ESVCP/ECVCP)

Lisbon, 30.08. – 02.09.2023

Quelle

Journal of comparative pathology

Bandzählung: 210

Seiten: 93

ISSN: 0021-9975

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S0021997524001749?via%3Dihub

DOI: 10.1016/j.jcpa.2024.03.145

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Hypersecretion and mucus plugging are hallmarks of equine asthma (EA), one of the most important respiratory diseases of horses. However, the proteins in the mucus with compositional changes in EA and their characteristics are widely unknown. The genomic characteristic and the tissue and cellular expression pattern of a molecule, which was abundantly detected in the mucus of horses with severe EA (SEA) for the first time, was further investigated in healthy horses (HHs).

Materials and methods: In a landscaping approach, the proteome of the airway mucus of HHs (n = 9) and horses with SEA (n = 10) was analysed using liquid chromatography tandem mass spectrometry. A molecule that was massively found in the mucus of SEA was genomically characterized in silico and the tissue and cellular expression pattern in the airways was analysed in HHs (n = 3) via RT-qPCR or in-situ hybridization, respectively.

Results: Vanin-1 was found abundantly in the mucus of horses affected by SEA compared with HHs. It is one of three vanin genes in the equine genome. All members were found to be expressed in the airway lining epithelium and submucosal glands along the respiratory tract, with Vanin-1 being predominantly expressed in tracheal as well as bronchial ciliated epithelial and goblet cells.

Conclusions: Vanin-1 is one of three members of a family which are expressed in the equine respiratory tract. It is a highly abundant protein in the mucus of horses with SEA. Its role in SEA will be further investigated.