Publikationsdatenbank

Repetitive exposure to bacteriophage cocktails against Pseudomonas aeruginosa or Escherichia coli provokes marginal humoral immunity in naïve mice (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Weissfuss, Chantal

Wienhold, Sandra-Maria

Bürkle, Magdalena

Gaborieau, Baptiste

Bushe, Judith (WE 12)

Behrendt, Ulrike

Bischoff, Romina

Korf, Imke H. E.

Wienecke, Sarah

Dannheim, Antonia

Ziehr, Holger

Rohde, Christine

Gruber, Achim D. (WE 12)

Ricard, Jean-Damien

Debarbieux, Laurent

Witzenrath, Martin

Nouailles, Geraldine

Quelle

Viruses

Bandzählung: 15

Heftzählung: 2

Seiten: Artikel 387

ISSN: 1999-4915

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1999-4915/15/2/387

DOI: 10.3390/v15020387

Pubmed: 36851601

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Phage therapy of ventilator-associated pneumonia (VAP) is of great interest due to the rising incidence of multidrug-resistant bacterial pathogens. However, natural or therapy-induced immunity against therapeutic phages remains a potential concern. In this study, we investigated the innate and adaptive immune responses to two different phage cocktails targeting either Pseudomonas aeruginosa or Escherichia coli-two VAP-associated pathogens-in naïve mice without the confounding effects of a bacterial infection. Active or UV-inactivated phage cocktails or buffers were injected intraperitoneally daily for 7 days in C57BL/6J wild-type mice. Blood cell analysis, flow cytometry analysis, assessment of phage distribution and histopathological analysis of spleens were performed at 6 h, 10 days and 21 days after treatment start. Phages reached the lungs and although the phage cocktails were slightly immunogenic, phage injections were well tolerated without obvious adverse effects. No signs of activation of innate or adaptive immune cells were observed; however, both active phage cocktails elicited a minimal humoral response with secretion of phage-specific antibodies. Our findings show that even repetitive injections lead only to a minimal innate and adaptive immune response in naïve mice and suggest that systemic phage treatment is thus potentially suitable for treating bacterial lung infections.