Publication Database

Characterization of Bacillus pumilus strains isolated from bovine uteri (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ballas, Panagiotis

Gabler, Christoph (WE 3)

Wagener, Karen

Drillich, Marc

Ehling-Schulz, Monika

Quelle

Animals

Bandzählung: 13

Heftzählung: 8

Seiten: Artikel 1297

ISSN: 2076-2615

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2615/13/8/1297

DOI: 10.3390/ani13081297

Pubmed: 37106860

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Uterine infections are a major source of economic losses to dairy farmers. The uterine microbiota as well as opportunistic uterine contaminants can contribute to the development of endometritis in dairy cows during the postpartum period. Therefore, it is important to characterize potential pathogens and to further elucidate their role in the disease. In this study, we aimed to characterize Bacillus pumilus field isolates to obtain more details regarding their effect on uterine cells by using an in vitro endometrial epithelial primary cells model. We found that B. pumilus isolates possessed the keratinase genes ker1 and ker2 and therefore may produce keratinases. When primary endometrial epithelial cells were infected with 4 different B. pumilus strains, an effect on cellular viability was observed over the course of 72 h. The effect was dose-dependent and time-dependent. Nevertheless, significant differences between the strains were not observed. All tested strains reduced the viability of the primary cells after 72 h of incubation, indicating that B. pumilus potentially has a pathogenic effect on endometrial epithelial cells.