Publikationsdatenbank

Immunohistochemical and molecular genetic analysis of canine digital mast cell tumours (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Conrad, David

Kehl, Alexandra

Müller, Tobias

Klopfleisch, Robert (WE 12)

Aupperle-Lellbach, Heike

Quelle

Animals

Bandzählung: 13

Heftzählung: 10

Seiten: Artikel 1694

ISSN: 2076-2615

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2615/13/10/1694

DOI: 10.3390/ani13101694

Pubmed: 37238124

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Grading, immunohistochemistry and c-kit mutation status are criteria for assessing the prognosis and therapeutic options of canine cutaneous mast cell tumours (MCTs). As a subset, canine digital MCTs have rarely been explored in this context. Therefore, in this retrospective study, 68 paraffin-embedded canine digital MCTs were analysed, and histological grading was assessed according to Patnaik and Kiupel. The immunohistochemical markers KIT and Ki67 were used, as well as polymerase chain reaction (PCR) for mutational screening in c-kit exons 8, 9, 11 and 14. Patnaik grading resulted in 22.1% grade I, 67.6% grade II and 10.3% grade III tumours. Some 86.8% of the digital MCTs were Kiupel low-grade. Aberrant KIT staining patterns II and III were found in 58.8%, and a count of more than 23 Ki67-positive cells in 52.3% of the cases. Both parameters were significantly associated with an internal tandem duplication (ITD) in c-kit exon 11 (12.7%). French Bulldogs, which tend to form well-differentiated cutaneous MCTs, had a higher proportion of digital high-grade MCTs and ITD in c-kit exon 11 compared with mongrels. Due to its retrospective nature, this study did not allow for an analysis of survival data. Nevertheless, it may contribute to the targeted characterisation of digital MCTs.