Publikationsdatenbank

Genomic characterization of Arcobacter butzleri strains isolated from various sources in Lithuania (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Uljanovas, Dainius

Gölz, Greta (WE 8)

Fleischmann, Susanne (WE 8)

Kudirkiene, Egle

Kasetiene, Neringa

Grineviciene, Audrone

Tamuleviciene, Egle

Aksomaitiene, Jurgita

Alter, Thomas (WE 8)

Malakauskas, Mindaugas

Quelle

Microorganisms : open access journal

Bandzählung: 11

Heftzählung: 6

Seiten: Artikel 1425

ISSN: 2076-2607

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2607/11/6/1425

DOI: 10.3390/microorganisms11061425

Pubmed: 37374927

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Arcobacter (A.) butzleri, the most widespread species within the genus Arcobacter, is considered as an emerging pathogen causing gastroenteritis in humans. Here, we performed a comparative genome-wide analysis of 40 A. butzleri strains from Lithuania to determine the genetic relationship, pangenome structure, putative virulence, and potential antimicrobial- and heavy-metal-resistance genes. Core genome single nucleotide polymorphism (cgSNP) analysis revealed low within-group variability (≤4 SNPs) between three milk strains (RCM42, RCM65, RCM80) and one human strain (H19). Regardless of the type of input (i.e., cgSNPs, accessory genome, virulome, resistome), these strains showed a recurrent phylogenetic and hierarchical grouping pattern. A. butzleri demonstrated a relatively large and highly variable accessory genome (comprising of 6284 genes with around 50% of them identified as singletons) that only partially correlated to the isolation source. Downstream analysis of the genomes resulted in the detection of 115 putative antimicrobial- and heavy-metal-resistance genes and 136 potential virulence factors that are associated with the induction of infection in host (e.g., cadF, degP, iamA), survival and environmental adaptation (e.g., flagellar genes, CheA-CheY chemotaxis system, urease cluster). This study provides additional knowledge for a better A. butzleri-related risk assessment and highlights the need for further genomic epidemiology studies in Lithuania and other countries.