Publikationsdatenbank

In vitro infection of primary chicken lymphocytes with Marek's disease virus (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Bertzbach, Luca D. (WE 5)

Kohn, Marina

You, Yu (WE 5)

Kossak, Lisa (WE 5)

Sabsabi, Mohammad A. (WE 5)

Kheimar, Ahmed (WE 5)

Härtle, Sonja

Kaufer, Benedikt B. (WE 5)

Quelle

STAR Protocols

Bandzählung: 4

Heftzählung: 2

Seiten: Artikel 102343

ISSN: 2666-1667

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2666166723003106

DOI: 10.1016/j.xpro.2023.102343

Pubmed: 37270781

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that infects immune cells and causes a deadly lymphoproliferative disease in chickens. Cytokines and monoclonal antibodies promote the survival of chicken lymphocytes in vitro. Here, we describe protocols for the isolation, maintenance, and efficient MDV infection of primary chicken lymphocytes and lymphocyte cell lines. This facilitates the investigation of key aspects of the MDV life cycle in the primary target cells of viral replication, latency, genome integration, and reactivation.