Publikationsdatenbank

Functionalized fullerene for inhibition of SARS-CoV-2 variants (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Page, Taylor M.

Nie, Chuanxiong

Neander, Lenard

Povolotsky, Tatyana L.

Sahoo, Anil Kumar

Nickl, Philip

Adler, Julia M. (WE 5)

Bawadkji, Obida

Radnik, Jörg

Achazi, Katharina

Ludwig, Kai

Lauster, Daniel

Netz, Roland R.

Trimpert, Jakob (WE 5)

Kaufer, Benedikt (WE 5)

Haag, Rainer

Donskyi, Ievgen S.

Quelle

Small : nano micro

Bandzählung: 19

Heftzählung: 15

Seiten: Artikel 2206154

ISSN: 1613-6829

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1002/smll.202206154

DOI: 10.1002/smll.202206154

Pubmed: 36651127

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

As virus outbreaks continue to pose a challenge, a nonspecific viral inhibitor can provide significant benefits, especially against respiratory viruses. Polyglycerol sulfates recently emerge as promising agents that mediate interactions between cells and viruses through electrostatics, leading to virus inhibition. Similarly, hydrophobic C60 fullerene can prevent virus infection via interactions with hydrophobic cavities of surface proteins. Here, two strategies are combined to inhibit infection of SARS-CoV-2 variants in vitro. Effective inhibitory concentrations in the millimolar range highlight the significance of bare fullerene's hydrophobic moiety and electrostatic interactions of polysulfates with surface proteins of SARS-CoV-2. Furthermore, microscale thermophoresis measurements support that fullerene linear polyglycerol sulfates interact with the SARS-CoV-2 virus via its spike protein, and highlight importance of electrostatic interactions within it. All-atom molecular dynamics simulations reveal that the fullerene binding site is situated close to the receptor binding domain, within 4 nm of polyglycerol sulfate binding sites, feasibly allowing both portions of the material to interact simultaneously.