Publikationsdatenbank

Two-photon excitation spectra of various fluorescent proteins within a broad excitation range (2022)

Art

Vortrag

Autoren

Leben, Ruth (WE 6)

Lindquist, Randall L.

Hauser, Anja E.

Niesner, Raluca (WE 2)

Rakhymzhan, Asylkhan

Kongress

32nd Meeting of the German Society for Cytometry (DGfZ)

Berlin, 28. – 30.09.2022

Quelle

International journal of molecular sciences

Bandzählung: 23

Heftzählung: 21

Seiten: Artikel 13407

ISSN: 1422-0067

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1422-0067/23/21/13407

DOI: 10.3390/ijms232113407

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Two-photon excitation fluorescence laser-scanning microscopy is the preferred method for studying dynamic processes in living organ models or even in living organisms. Thanks to near-infrared and infrared excitation, it is possible to penetrate deep into tissue, reaching areas of interest for life sciences
and biomedicine. In those imaging experiments, two-photon excitation spectra are needed to select the optimal laser wavelength to excite as many fluorophores as possible simultaneously in the sample under consideration. The more fluorophores that can be excited, the more cell populations that can be studied, and the better the access to their arrangement and interaction in complex systems such as lymphoid organs (Rakhymzhan et al. 2017). However, the two-photon excitation properties are poorly predicted by the single-photon spectra and are not yet available in literature or in databases, for many fluorophores (Ricard et al. 2018). Here we present the broad excitation range (760 nm to 1300 nm) photon fluxnormalized two-photon spectra of several fluorescent proteins in their cellular environment. This includes following fluorescent proteins: mCerulean3, mTurquoise2, mT-Sapphire, Clover, mKusabiraOrange2, mOrange2, LSS-mOrange, mRuby2, mBeRFP, mCardinal, iRFP670, NirFP, iRFP720, ranging from blue to red and even infrared fluorescence