Publikationsdatenbank

Multidrug-resistance mediating integrative and conjugative elements in Pasteurellaceae isolates from Germany (2022)

Art

Poster

Autoren

Schink, A.-K. (WE 7)

Hanke, D. (WE 7)

Semmler, T.

Brombach, J. (WE 7)

Bethe, A. (WE 7)

Lübke-Becker, A. (WE 7)

Teske, K. (WE 12)

Müller, K. E. (WE 18)

Schwarz, S. (WE 7)

Kongress

ISAH 2022 : 20th Congress of the International Society for Animal Hygiene

Berlin, 05. – 07.10.2022

Quelle

ISAH 2022 : 20th congress of the international society for animal hygiene : 5.–7. October 2022 — Freie Universität Berlin, Fachbereich Veterinärmedizin Institut für Tier- und Umwelthygien (Hrsg.)

Berlin, 2022 — S. 126–127

Sprache

Englisch

Verweise

URL (Volltext): https://www.vetmed.fu-berlin.de/en/einrichtungen/institute/we10/ISAH-2022/_downloads/Abstract-Book-ISAH2022.pdf

Kontakt

Nutztierklinik

Königsweg 65
14163 Berlin
+49 30 838 62261
klauentierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Integrative and conjugative elements (ICEs) are mobile genetic elements, which facilitate the spread of antimicrobial resistance genes among bacteria. In doing so, they are not limited to bacteria of a specific genus, but might be transferred between bacteria of different genera or even beyond bacterial family borders. Pasteurellaceae isolates carrying ICEs have been reported from the USA and Canada. Therefore, a Mannheimia haemolytica and a Pasteurella multocida isolate from Germany, which play important roles in the pathogenesis of the bovine respiratory disease (BRD) complex, with rarely occurring phenotypic multidrug-resistance (MDR) were analysed to determine (i) the antimicrobial resistance genes present, (ii) their potential association with mobile genetic elements and (iii) mutations within the genes of antimicrobial target structures.

Material & Methods:
In November 2019, the isolates M. haemolytica IMT47952 and P. multocida IMT47951 were identified during routine diagnostics in lung samples taken at necropsy from a severe case of BRD of a three-month-old German calf. The antimicrobial susceptibilities of M. haemolytica IMT47952 and P. multocida IMT47951 were determined according to CLSI standards. Hybrid assembly of MiSeq and MinION reads resulted in closed genomes of both isolates.

Results:
Both isolates showed MDR phenotypes. Analysis of the M. haemolytica IMT47952 sequence revealed the presence of the resistance genes sul2 [sulfonamides], catA3 [chloramphenicol], floR [chloramphenicol/florfenicol], tet(Y) [tetracyclines], strA-strB [streptomycin], mef(C) and mph(G) [macrolides] as part of the novel ICE Tn₇₄₀₆. In addition, nucleotide exchanges leading to amino acid substitutions D87Y in GyrA [nalidixic acid] and W30R in FolA [trimethoprim] were identified. In the sequence of P. multocida IMT47951, the resistance genes sul2, strA-strB, tet(H) [tetracyclines] and aphA1 [kanamycin, neomycin] were located within the novel ICE Tn₇₄₀₇. Furthermore, an A2059C transition in the 23S rRNA of all six rRNA operons might explain the elevated minimal inhibitory concentration values for macrolides and clindamycin.

Discussion:
The resistance genes tet(Y), mef(C) and mph(G) have been identified in bacteria of aquatic origin, but not yet in M. haemolytica. Moreover, the first identification of multidrug-resistance mediating ICEs in respiratory tract pathogens in Germany is alarming as these elements may diminish treatment options for BRD in the future and pose a risk not only for animal, but also for human health, if transferred to other bacterial pathogens, via the food chain or established in an environmental reservoir.