Publikationsdatenbank

Salmonella central carbon metabolism enhances bactericidal killing by fluoroquinolone antibiotics (2022)

Art

Poster

Autoren

Braetz, Sebastian (WE 7)

Schwerk, Peter (WE 7)

Thompson, Arthur

Tedin, Karsten (WE 7)

Fulde, Marcus (WE 7)

Kongress

Zoonoses 2022 - International Symposium on Zoonoses Research

Berlin, 05. – 07.10.2022

Quelle

Antimicrobial agents and chemotherapy

Bandzählung: 66

Heftzählung: 7

Seiten: Artikel e0234421

ISSN: 1098-6596

Sprache

Englisch

Verweise

URL (Volltext): https://journals.asm.org/doi/10.1128/aac.02344-21

DOI: 10.1128/aac.02344-21

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The efficacy of killing by bactericidal antibiotics has been reported to depend in large part on the ATP levels, with low levels of ATP leading to increased persistence after antibiotic challenge. Here, we show that an atp operon deletion strain of Salmonella enterica serovar Typhimurium lacking the ATP synthase was at least 10-fold more sensitive to killing by the fluoroquinolone antibiotic ciprofloxacin and yet showed either increased survival or no significant difference compared with the wild-type strain when challenged with aminoglycoside or β-lactam antibiotics, respectively. The increased cell killing and reduced bacterial survival (persistence) after fluoroquinolone challenge were found to involve metabolic compensation for the loss of the ATP synthase through central carbon metabolism reactions and increased NAD(P)H levels. We conclude that the intracellular ATP levels per se do not correlate with bactericidal antibiotic persistence to fluoroquinolone killing; rather, the central carbon metabolic pathways active at the time of challenge and the intracellular target of the antibiotic determine the efficacy of treatment.