Publikationsdatenbank

Fluorescence based approach to quantify the viability of Ascaris suum L3 larvae (2022)

Art

Vortrag

Autor

Kundik, A. (WE 6)

Kongress

Tagung der DVG-Fachgruppe Parasitologie und parasitäre Krankheiten

Berlin & online, 23. – 25.05.2022

Quelle

Tagung der DVG-Fachgruppe Parasitologie und parasitäre Krankheiten : 23. bis 25. Mai 2022 Hybrid-Tagung (Berlin & Online) — DVG Deutsche Veterinärmedizinische Gesellschaft e.V. ; wissenschaftliche Leitung: Prof. Dr. Georg von Samson-Himmelstjerna, Berlin (Hrsg.)

1. Auflage

Gießen: Verlag der DVG Service GmbH, 2022 — S. 113–114

ISBN: 978-3-86345-624-5

Sprache

Englisch

Verweise

URL (Volltext): https://www.dvg.net/fileadmin/Bilder/DVG/PDF/Gesamtdatei_Tagungsband_Parasitologie_2022.pdf

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Due to the concern of arising anthelmintic resistances the demand for new drugs against helminth parasites is rising. Unfortunately, drug screening assays like the migration and motility assay are time consuming, and prone to individual subjectivity; Moreover, both methods do not provide information on the metabolic activity. For this reason, we are evaluating an in vitro assay using the fluorescence dye resazurin. The assay is conducted in a 96-well plate with at least 100 Ascaris suum L3 larvae per well and the florescence signal is measured after 24 h with a plate reader. According to our preliminary data, the dye is taken up by vital larvae, reduced to a fluorescence metabolite, and released back into the media. This assay can be possibly applied to complement the usual anthelmintic drug screening approaches with additional information on metabolic potential of helminth.