Publikationsdatenbank

mcr-1 colistin resistance gene sharing between Escherichia coli from cohabiting dogs and humans, Lisbon, Portugal, 2018 to 2020 (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Menezes, Juliana

Moreira da Silva, Joana

Frosini, Sian-Marie

Loeffler, Anette

Weese, Scott

Perreten, Vincent

Schwarz, Stefan (WE 7)

Telo da Gama, Luís

Amaral, Andreia Jesus

Pomba, Constança

Quelle

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

Bandzählung: 27

Heftzählung: 44

Seiten: Artikel 2101144

ISSN: 1560-7917

Sprache

Englisch

Verweise

URL (Volltext): https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2022.27.44.2101144

DOI: 10.2807/1560-7917.ES.2022.27.44.2101144

Pubmed: 36330821

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background The emergence of colistin resistance is a One Health antimicrobial resistance challenge worldwide. The close contact between companion animals and humans creates opportunities for transmission and dissemination of colistin-resistant bacteria.AimTo detect potential animal reservoirs of colistin-resistant Escherichia coli and investigate the possible sharing of these bacteria between dogs, cats and their cohabiting humans in the community in Lisbon, Portugal.MethodsA prospective longitudinal study was performed from 2018 to 2020. Faecal samples from dogs and cats either healthy or diagnosed with a skin and soft tissue or urinary tract infection, and their cohabiting humans were screened for the presence of colistin-resistant E. coli. All isolates were tested by broth microdilution against colistin and 12 other antimicrobials. Colistin-resistant isolates were screened for 30 resistance genes, including plasmid-mediated colistin resistance genes (mcr-1 to mcr-9), and typed by multilocus sequence typing. Genetic relatedness between animal and human isolates was analysed by whole genome sequencing.ResultsColistin-resistant E. coli strains harbouring the mcr-1 gene were recovered from faecal samples of companion animals (8/102; 7.8%) and humans (4/125; 3.2%). No difference between control and infection group was detected. Indistinguishable multidrug-resistant E. coli ST744 strains harbouring the mcr-1 gene were found in humans and their dogs in two households.ConclusionsThe identification of identical E. coli strains containing the plasmid-mediated mcr-1 gene in companion animals and humans in daily close contact is of concern. These results demonstrate the importance of the animal-human unit as possible disseminators of clinically important resistance genes in the community setting.