Publication Database

Characterization of a genomic Island carrying the tet(X4) gene in porcine Acinetobacter towneri co-harboring plasmid-borne bla_NDM-1 and bla_OXA-58 genes (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Li, Aijuan

Yu, Runhao

Zhao, Wenbo

Schwarz, Stefan (WE 7)

Li, Chenglong

Yao, Hong

Du, Xiang-Dang

Quelle

Frontiers in veterinary science : FVETS

Bandzählung: 9

Seiten: Artikel 1002149

ISSN: 2297-1769

Sprache

Englisch

Verweise

URL (Volltext): https://www.frontiersin.org/articles/10.3389/fvets.2022.1002149/full

DOI: 10.3389/fvets.2022.1002149

Pubmed: 36246313

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Tigecycline and carbapenems are last-resort antimicrobial agents to treat serious infections caused by multi-drug resistant bacterial pathogens. However, the co-occurrence of tigecycline and carbapenem resistance determinants challenges the clinical efficacy of these antimicrobial agents. In this study, we report the co-existence of tet(X4), bla_NDM-1 and bla_OXA-58 genes in the porcine Acinetobacter towneri isolate 19110F47. Sequence analysis revealed that tet(X4) gene, along with the florfenicol resistance gene floR, was flanked by three copies of IS91-like elements, which can form three different translocatable units (TUs), and were located in a 41,098-bp multidrug resistance region (MDRR) within a novel 100,354-bp genomic island (GI) region. TUs comprising floR-virD2-ISVsa3, hp-abh-tet(X4)-ISVsa3 and virD2-floR-ISVsa3-hp-abh-tet(X4)-ISVsa3 can be looped out from the chromosomal DNA and facilitate the transfer of the TU-based resistance genes into other plasmidic or chromosomal sites. In addition, the carbapenemase genes bla_NDM-1 and bla_OXA-58 were found on different non-conjugative multiresistance plasmids in this isolate, with the genetic contexts ISAba125-bla_NDM-1-ble_MBL-tnpR and ΔISAba3-bla_OXA-58-ISAba3, respectively. The simultaneous occurrence of tet(X4), bla_NDM-1 and bla_OXA-58 in the same porcine A. towneri isolate emphasizes the importance of antimicrobial resistance surveillance in food-producing animals.