Publication Database

Cellular distribution pattern of tjp1 (ZO-1) in Xenopus laevis oocytes heterologously expressing claudins (2023)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Brunner, Nora (WE 2)

Stein, Laura (WE 2)

Amasheh, Salah (WE 2)

Quelle

The journal of membrane biology : an internat. journal for studies on the structure, function and genesis of biomembranes

Bandzählung: 256

Heftzählung: 1

Seiten: 51 – 61

ISSN: 0022-2631

Sprache

Englisch

Verweise

URL (Volltext): https://link.springer.com/article/10.1007/s00232-022-00251-z

DOI: 10.1007/s00232-022-00251-z

Pubmed: 35737002

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Epithelial barriers constitute a fundamental requirement in every organism, as they allow the separation of different environments and set boundaries against noxious and other adverse effectors. In many inflammatory and degenerative diseases, epithelial barrier function is impaired because of a disturbance of the paracellular seal. Recently, the Xenopus laevis oocyte has been established as a heterologous expression model for the analysis of transmembrane tight junction protein interactions and is currently considered to be a suitable screening model for barrier effectors. A prerequisite for this application is a physiological anchoring of claudins to the cytoskeleton via the major scaffolding protein tjp1 (tight junction protein 1, ZO-1). We have analyzed the oocyte model with regard to the interaction of heterologously expressed claudins and tjp1. Our experiments have revealed endogenous tjp1 expression in protein and mRNA analyses of unfertilized Xenopus laevis oocytes expressing human claudin 1 (CLDN1) to claudin 5 (CLDN5). The amphibian cell model can therefore be used for the analysis of claudin interactions.