Publikationsdatenbank

Development of new methods to reduce microbial contaminants during chicken slaughtering (2022)

Art

Poster

Autoren

Carstens, G. (WE 10)

Friese, A. (WE 10)

Roesler, U. (WE 10)

Kongress

ISAH 2022 : 20th Congress of the International Society for Animal Hygiene

05. – 07.10.2022

Quelle

ISAH 2022 : 20th Congress of the International Society for Animal Hygiene — Freie Universität Berlin (Hrsg.)

Berlin, 2022 — S. 165–166

Sprache

Englisch

Verweise

URL (Volltext): https://www.vetmed.fu-berlin.de/en/einrichtungen/institute/we10/ISAH-2022/_downloads/Abstract-Book-ISAH2022.pdf

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction
Intervention measures to prevent humans from foodborne illnesses are implemented in the entire broiler production chain. As these measures are primarily taken in the pre-harvest production, cross contamination at slaughterhouse level is commonly detected. We aimed to optimize existing procedures and develop new methods to reduce microbial contaminants in chicken slaughtering. For this, we focused on (I) process water and (II) ambient air in the slaughterhouse and their role in terms of cross contamination during the slaughter process.
Material & Methods
I) The efficacy of organic acids (formic acid and lactic acid) and oxygen releasing agents (peracetic acid) was tested in suspension (DIN EN 1276) on different bacterial pathogens (Enterococcus hirae, Staphylococcus aureus, Salmonella Typhimurium) using autoclaved scalding water from a broiler slaughterhouse as organic soiling. Based on the scalding process, a testing temperature of 52°C and an exposure time of 3 minutes was chosen.
II) We evaluated the bacterial load in the ambient air by taking samples at different process steps and at multiple times in a broiler slaughterhouse using two Coriolis µ cyclonic air sampler. We tested the efficacy of the preinstalled measures ozonation, UV-C irradiation and ionization to reduce airborne pathogens by taking samples over the course of a day with and without these measures in operation.
Results
I) The tested disinfectants showed a 5-log reduction of the investigated bacterial pathogens using concentrations ranging of 0.001% to 0.004% for peracetic acid, 0.04% to 0.5% for formic acid and 0.1% to 2% for lactic acid. E. hirae was the limiting pathogen in all experimental setups, requiring the highest concentrations of disinfectants for a 5-log reduction.
II) The investigated air samples from the slaughterhouse showed a bacterial load in the processing area with quantities ranging from < 1x101 cfu/m³ to 3.3x10³ cfu/m³. During the course of a day, bacterial load increased significantly in the air. No differences in airborne bacterial load were shown using ozonation, UVC irradiation or ionization in the processing area of the slaughterhouse.
Discussion
I) Our results demonstrate that an effective disinfection in suspension is possible with practical relevant very low concentrations for the three tested disinfectants. For an implementation of adding these disinfectants to the scalding water during slaughter process, the impact of disinfectants on carcasses needs to be evaluated subsequently.
II) The results of our ambient air samplings indicate a low bacterial load in the processing area of broiler slaughterhouses. The rise of airborne bacteria during the course of the day was to be expected due to the continuous slaughter process and increasing number of passing carcasses. However, ozonation, UVC irradiation and ionization are non-effective measures to decrease bacterial load in this setup.
Acknowledgement & Funding
KontRed Project-Funding: Federal Ministry of Food and Agriculture (funding number: 281C104B18)