Publikationsdatenbank

Sequence analysis of novel Staphylococcus aureus lineages from wild and captive macaques (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Monecke, Stefan

Roberts, Marilyn C.

Braun, Sascha D.

Diezel, Celia

Müller, Elke

Reinicke, Martin

Linde, Jörg

Joshi, Prabhu Raj

Paudel, Saroj

Acharya, Mahesh

Chalise, Mukesh K.

Feßler, Andrea T. (WE 7)

Hotzel, Helmut

Khanal, Laxman

Koju, Narayan P.

Schwarz, Stefan (WE 7)

Kyes, Randall C.

Ehricht, Ralf

Quelle

International journal of molecular sciences

Bandzählung: 23

Heftzählung: 19

Seiten: Artikel 11225

ISSN: 1422-0067

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1422-0067/23/19/11225

DOI: 10.3390/ijms231911225

Pubmed: 36232529

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Staphylococcus aureus is a widespread and common opportunistic bacterium that can colonise or infect humans as well as a wide range of animals. There are a few studies of both methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolated from monkeys, apes, and lemurs, indicating a presence of a number of poorly or unknown lineages of the pathogen. In order to obtain insight into staphylococcal diversity, we sequenced strains from wild and captive individuals of three macaque species (Macaca mulatta, M. assamensis, and M. sylvanus) using Nanopore and Illumina technologies. These strains were previously identified by microarray as poorly or unknown strains. Isolates of novel lineages ST4168, ST7687, ST7688, ST7689, ST7690, ST7691, ST7692, ST7693, ST7694, ST7695, ST7745, ST7746, ST7747, ST7748, ST7749, ST7750, ST7751, ST7752, ST7753, and ST7754 were sequenced and characterised for the first time. In addition, isolates belonging to ST2990, a lineage also observed in humans, and ST3268, a MRSA strain already known from macaques, were also included into the study. Mobile genetic elements, genomic islands, and carriage of prophages were analysed. There was no evidence for novel host-specific virulence factors. However, a conspicuously high rate of carriage of a pathogenicity island harbouring edinB and etD2/etE as well as a higher number of repeat units within the gene sasG (encoding an adhesion factor) than in human isolates were observed. None of the strains harboured the genes encoding Panton-Valentine leukocidin. In conclusion, wildlife including macaques may harbour an unappreciated diversity of S. aureus lineages that may be of clinical relevance for humans, livestock, or for wildlife conservation, given the declining state of many wildlife populations.