Publication Database

Metabolic characteristics of porcine LA-MRSA CC398 and CC9 isolates from Germany and China via Biolog Phenotype MicroArrayTM (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Krüger-Haker, Henrike (WE 7)

Ji, Xing

Bartel, Alexander

Feßler, Andrea T. (WE 7)

Hanke, Dennis (WE 7)

Jiang, Nansong

Tedin, Karsten (WE 7)

Maurischat, Sven

Wang, Yang

Wu, Congming

Schwarz, Stefan (WE 7)

Quelle

Microorganisms : open access journal

Bandzählung: 10

Heftzählung: 11

Seiten: Artikel 2116

ISSN: 2076-2607

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2607/10/11/2116

DOI: 10.3390/microorganisms10112116

Pubmed: 36363707

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is an important zoonotic pathogen, often multi-resistant to antimicrobial agents. Among swine, LA-MRSA of clonal complex (CC) 398 dominates in Europe, Australia and the Americas, while LA-MRSA-CC9 is the main epidemic lineage in Asia. Here, we comparatively investigated the metabolic properties of rare and widespread porcine LA-MRSA isolates from Germany and China using Biolog Phenotype MicroArray technology to evaluate if metabolic variations could have played a role in the development of two different epidemic LA-MRSA clones in swine. Overall, we were able to characterize the isolates’ metabolic profiles and show their tolerance to varying environmental conditions. Sparse partial least squares discriminant analysis (sPLS-DA) supported the detection of the most informative substrates and/or conditions that revealed metabolic differences between the LA-MRSA lineages. The Chinese LA-MRSA-CC9 isolates displayed unique characteristics, such as a consistently delayed onset of cellular respiration, and increased, reduced or absent usage of several nutrients. These possibly unfavorable metabolic properties might promote the ongoing gradual replacement of the current epidemic LA-MRSA-CC9 clone in China with the emerging LA-MRSA-CC398 lineage through livestock trade and occupational exposure. Due to the enhanced pathogenicity of the LA-MRSA-CC398 clone, the public health risk posed by LA-MRSA from swine might increase further.