Publikationsdatenbank

Correlation of intestinal amino acid flux rates in the small intestine of pigs receiving different methionine supplements (2022)

Art

Poster

Autoren

Schermuly, I. I. (WE 2)

Romanet, S. (WE 2)

Patra, A. K.

Klünemann, M.

Whelan, R. A.

Aschenbach, J. R. (WE 2)

Kongress

Tagung der Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft (DVG)

Gießen, 24. – 26.06.2022

Quelle

Sprache

Englisch

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Methionine (Met) is an essential and limiting amino acid (AA) in pigs. Putative apical transporters for Met are either Na+-dependent or Na+-independent and accept also other AA. A possible regulation of Met uptake by dietary Met supplements might thus also influence the uptake of other AA. The aim
of the present study was to elucidate the relationship between the intestinal flux of ten selected AA in pigs fed three different Met supplements to conclude on the possibly involved transporters.
Methods: Forty stably weaned pigs received a basal diet that had reduced concentration of sulfur AA (0.26% Met, 0.30% Cys). The sulfur AA-deficient diet was supplemented in three groups with one of the following additives: 0.21% L-Met, 0.21% DL-Met or 0.31% DL-2-hydroxy-4-(methylthio)butanoic
acid (DL-HMTBA). One group did not receive a Met supplement. Mucosal-to-serosal AA fluxes of 14C- labelled AA (Gly, L-Gln, L-Leu, L-Lys, L-Met, L-Ser, L-Thr, L-Trp, L-Tyr, L-Val) were measured in the presence and absence of mucosal Na+ at an AA concentration of 50 μM in the Ussing chamber. The
mean values of two flux periods of 45 min each were used for statistical analyses by two-way ANOVA. The Na+-dependent AA flux rate was computed by subtracting the flux rate in the absence of Na+ from the flux rate in the presence of Na+ and submitted to Pearson correlation analysis.
Results: All AA showed higher flux rates in the presence vs. absence of mucosal Na+ (P < 0.05) with no effect of diet. The Na+-dependent AA flux rates showed several correlations to each other. The highest correlations were found between Tyr–Lys (r = 0.70) and Tyr–Ser (r = 0.79). High correlations
also existed between Gln–Leu (r = 0.61), Gln–Val (r = 0.59), Met–Val (r = 0.55), Met–Ser (r = 0.58), Tyr–Thr (r = 0.58), Thr–Gln (r = 0.51), Ser-Lys (r = 0.56) and Lys–Thr (r = 0.52). Only Trp was not correlated to any other AA and Gly was only correlated with Thr (r = 0.44).
Conclusions: Under the applied conditions, absorption of all tested AA acids was partially Na+- dependent and not influenced by diet. The high correlation pattern among the Na+-dependent portion of diverse neutral AA suggests the involvement of at least one Na+-dependent transport system with broader specificity for neutral AA and low or missing affinity for Gly. The correlations between the cationic AA Lys and some neutral AA point to the involvement of a Na+-dependent transport system with affinity for both cationic and neutral AA. The missing correlations between Trp and all other AA requires further investigations.
This study was supported by Evonik Nutrition & Care GmbH and an Elsa Neumann Grant of the State of Berlin.