Publication Database

Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ivanusic, Daniel

Madela, Kazimierz

Bannert, Norbert

Denner, Joachim (WE 5)

Quelle

MicroPublication : Biology

Bandzählung: 2022

Seiten: Artikel 000648

ISSN: 2578-9430

Sprache

Englisch

Verweise

URL (Volltext): https://www.micropublication.org/journals/biology/micropub-biology-000648

DOI: 10.17912/micropub.biology.000648

Pubmed: 36281316

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1).