Publikationsdatenbank

Pig-to-non-human primate heart transplantation:
the final step toward clinical xenotransplantation? (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Reichart, Bruno

Längin, Matthias

Radan, Julia

Mokelke, Maren

Buttgereit, Ines

Ying, Jiawei

Fresch, Ann Kathrin

Mayr, Tanja

Issl, Lara

Buchholz, Stefan

Michel, Sebastian

Ellgass, Reinhard

Mihalj, Maks

Egerer, Stefanie

Baehr, Andrea

Kessler, Barbara

Kemter, Elisabeth

Kurome, Mayuko

Zakhartchenko, Valeri

Steen, Stig

Sjöberg, Trygve

Paskevicius, Audrius

Krüger, Luise

Fiebig, Uwe

Denner, Joachim (WE 5)

Godehardt, Antonia W.

Tönjes, Ralf R.

Milusev, Anastasia

Rieben, Robert

Sfriso, Riccardo

Walz, Christoph

Kirchner, Thomas

Ayares, David

Lampe, Karen

Schönmann, Uwe

Hagl, Christian

Wolf, Eckhard

Klymiuk, Nikolai

Abicht, Jan-Michael

Brenner, Paolo

Quelle

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation

Bandzählung: 39

Heftzählung: 8

Seiten: 751 – 757

ISSN: 1053-2498

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/abs/pii/S1053249820315564

DOI: 10.1016/j.healun.2020.05.004

Pubmed: 32527674

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
The demand for donated human hearts far exceeds the number available. Xenotransplantation of genetically modified porcine organs provides an alternative. In 2000, an Advisory Board of the International Society for Heart and Lung Transplantation set the benchmark for commencing clinical cardiac xenotransplantation as consistent 60% survival of non-human primates after life-supporting porcine heart transplantations. Recently, we reported the stepwise optimization of pig-to-baboon orthotopic cardiac xenotransplantation finally resulting in consistent success, with 4 recipients surviving 90 (n = 2), 182, and 195 days. Here, we report on 4 additional recipients, supporting the efficacy of our procedure.

Results:
The first 2 additional recipients succumbed to porcine cytomegalovirus (PCMV) infections on Days 15 and 27, respectively. In 2 further experiments, PCMV infections were successfully avoided, and 3-months survival was achieved. Throughout all the long-term experiments, heart, liver, and renal functions remained within normal ranges. Post-mortem cardiac diameters were slightly increased when compared with that at the time of transplantation but with no detrimental effect. There were no signs of thrombotic microangiopathy. The current regimen enabled the prolonged survival and function of orthotopic cardiac xenografts in altogether 6 of 8 baboons, of which 4 were now added. These results exceed the threshold set by the Advisory Board of the International Society for Heart and Lung Transplantation.

Conclusions:
The results of our current and previous experimental cardiac xenotransplantations together fulfill for the first time the pre-clinical efficacy suggestions. PCMV-positive donor animals must be avoided.