Publication Database

Impact of feed additives and host-related factors on bacterial metabolic activity, and immunological traits in the caecum of broilers (2022)

Art

Poster

Autoren

Duangnumsawang, Yada (WE 4)

Goodarzi Boroojeni, Farshad (WE 4)

Vahjen, Wilfried (WE 4)

Tarradas, Joan

Esteve-Garcia, Enric

Alberdi, Antton

Marcos, Sofia

Zentek, Jürgen (WE 4)

Kongress

1st Applied HoloGenomics Conference

Bilbao, Spanien, 13. – 15.10.2022

Quelle

Presentation abstracts for the 1st Applied HoloGenomics Conference — HoloFood Innovation Action (Hrsg.)

— S. 22

Sprache

Englisch

Verweise

URL (Volltext): https://www.holofood.eu/AHC_Abstract_Book.pdf

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

A total of 2,880 one-day-old male and female broiler chicks from Ross and Cobb were randomly allocated to 72 pens. Broilers were fed a wheat-soybean diet without (control) or with a probiotic (Bacillus subtilis and B. amyloliquefaciens) or phytobiotic (grape extract) supplement. One chicken per pen was selected at day (d) 7, 21, 35 of age for caecal sampling.
Data were subjected to ANOVA with a 3×3×2×2 (diet, age, breed and sex) factorial arrangement. Spearman’s correlation (r) was analyzed between bacterial metabolites and gene expressions. Overall, short-chain fatty acids (SCFAs) concentration increased with age (p < 0.05). L- and D-lactate decreased from d 7 to 21 (p < 0.05) and stayed constant until d 35.
Expression of interleukin (IL)-2, 4, 6, 8, 10, 12, 17, 18 as well as IFN-γ and TGF-β2 increased with age, but IL-1β and TNF-α increased from d 7 to 21, then declined (p < 0.05). Expression of MUC2 decreased from d 7 to 21, and then increased afterward, while expression of CLDN5 increased from d 21 to 35 (p < 0.05). Cobb showed higher D-lactate concentration and IL-1β, IL-6, TNF-α and CLDN5 expressions than Ross (p < 0.05). Probiotic diet showed higher IL-10 expression compared to the control (p < 0.05). At d 7 and 21, SCFAs and lactate were negatively correlated with pro-inflammatory cytokines e.g., IL-18 (r = -0.51 to -0.24) and TNF-α (r = -0.37 to -0.24), but these correlations almost disappeared at d 35. Age influenced all variables measured. A few effects of diet and breed on the variables measured showed no systematic biological pattern, while the influence of sex and interactions between factors was obscured. Age-related alterations in bacterial metabolites may affect immunological response in the caecum of broilers.