Publikationsdatenbank

Impact of feed additives and host-related factors on bacterial metabolic activity, and immunological traits in the ileum of broilers (2022)

Art

Poster

Autoren

Duangnumsawang, Yada (WE 4)

Goodarzi Boroojeni, Farshad (WE 4)

Vahjen, Wilfried (WE 4)

Tarradas, Joan

Esteve-Garcia, Enric

Alberdi, Antton

Marcos, Sofia

Zentek, Jürgen (WE 4)

Kongress

1st Applied HoloGenomics Conference

Bilbao, Spanien, 13. – 15.09.2022

Quelle

Presentation abstracts for the 1st Applied HoloGenomics Conference — HoloFood Innovation Action (Hrsg.)

— S. 21

Sprache

Englisch

Verweise

URL (Volltext): https://www.holofood.eu/AHC_Abstract_Book.pdf

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

One-day old male and female chicks from Ross (1,440 chicks) and Cobb (1,440 chicks) were randomly assigned to 72 pens. Broilers were offered a wheat-soybean diet without (control) or with a probiotic (Bacillus subtilis and B. amyloliquefaciens) or phytobiotic (grape extract) supplement. One chicken per pen was selected at day (d) 7, 21, 35 of age for ileal sampling.
Data were subjected to ANOVA with a 3×3×2×2 (diet, age, breed and sex) factorial arrangement. Spearman’s correlation (r) was analyzed between bacterial metabolites and gene expressions. Overall, at d 21, the concentration of short chain fatty acids (SCFAs) reached the peak, while lactate decreased to its lowest level (p < 0.05). Breed affected D-lactate, with a higher concentration in Ross than Cobb (p < 0.05). Expression of all genes including interleukin (IL)-1β, 2, 4, 6, 8, 10, 12, 17, 18 as well as TNF-α, IFN-γ, TGF-β2, MUC2 and CLDN5 increased from d 7 to 21 but decreased from d 21 to 35 (p < 0.05). Broilers fed phytobiotic and control diets had higher IL-1β expression than those fed probiotic diet (p < 0.05). Cobb showed higher
expression of IL-4, IL-6 and TNF-α, while Ross showed greater IFN-γ expression (p < 0.05). The influence of sex and interactions between factors on measured variables was obscured (p > 0.05). SCFAs showed negative correlations with pro-inflammatory cytokines at d 7 (r = -0.41 to -0.24) but displayed positive correlations with them at d 21 (r = 0.25 to 0.59). The correlations almost disappeared at d 35. Lactate showed negative correlations with pro-inflammatory cytokines at only d 7 and 35 (r = -0.27 to -0.23). Alterations in the evaluated bacterial metabolites by age may affect gut immune response in the ileum of broilers.