Publikationsdatenbank

Bovine mammary epithelial cells in vitro:
the future of milk production? (2022)

Art

Poster

Autoren

Bonack, Annekatrin (WE 1)

Plendl, Johanna (WE 1)

Al Masri, Salah (WE 1)

Kongress

116th Annual Meeting of the Anatomische Gesellschaft : joint meeting with the Anatomical Society

Berlin, 20. – 23.09.2022

Quelle

Abstract book : 116th annual meeting of the Anatomische Gesellschaft : joint meeting with the Anatomical Society — Charité - Universitätsmedizin Berlin (Hrsg.)

— S. 24

Sprache

Englisch

Verweise

URL (Volltext): https://anatomical.aey-congresse.de/index/program.html?file=files/anatomical/202209_Berlin_116th-Meeting_AbstractBook.pdf&cid=10390

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Whilst in vitro production of meat is well advanced this is not the case for milk yet. Even plant-based alternatives’ climate impact is bigger than expected. Could in vitro milk production be a possible solution? Aim of this study is to investigate the possibilities of in vitro milk production using bovine mammary epithelial cell cultures.

Samples of bovine mammary glands were enzymatically digested. Tissue was put through a cell strainer and single cells were seeded in cell culture dishes. Cells were incubated at 37°C, 5% CO₂ in basal medium. Later medium was changed to induction medium containing hormones to induce production of milk ingredients. For identification of cells immunohistochemistry was performed using EpCam, KRT18 and Vimentin as primary antibodies.

After two days of incubation primary cells formed a cobblestone-like confluent layer covering the cell culture dish. Cells in induction medium also developed cobblestone-like patterns. Whereas cells in basal medium kept their cobblestone-like morphology, cells in induction medium developed cellular heterogeneity, lumen-like structures and vesicle-like structures in varying sizes and shapes inside the cytoplasm. Cells in both media stained positive for EpCam and KRT 18 and negative for vimentin.

Different cell culture media cause varying cell morphology. Only cells incubated in induction medium developed lumen-like structures. Therefore, cells seem to begin to express cell polarity which later will be critical for mechanisms of milk secretion. The production of milk components (proteins and lipids) is under investigation using transmission electronmicroscopy, PCR and Western Blot.