Publication Database

Development of quality control ranges for biocide susceptibility testing (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schug, Angela R. (WE 7)

Scholtzek, Anissa D. (WE 7)

Turnidge, John

Meurer, Marita

Schwarz, Stefan (WE 7)

Feßler, Andrea T. (WE 7)

Grobbel, Mirjam

Tenhagen, Bernd-Alois

Kaspar, Heike

Kluge, Maria

Akhda, Silke

Mattauch, Marie-Christin (WE 10)

Friese, Anika (WE 10)

Roesler, Uwe (WE 10)

Kramer, Axel

Zwicker, Paula

Freitag, Christin

Kadlec, Kristina

Assmann, Julia

Walther, Birgit

Cuny, Christiane

Hölzle, Ludwig

Quelle

Pathogens

Bandzählung: 11

Heftzählung: 2

Seiten: Artikel 223

ISSN: 2076-0817

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-0817/11/2/223

DOI: 10.3390/pathogens11020223

Pubmed: 35215165

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The Biocide Susceptibility Study Group:
Mirjam Grobbel, Bernd-Alois Tenhagen (Department of Biological Safety, German Federal Institute for Risk Assessment (BfR), Germany), Heike Kaspar, Maria Kluge (Federal Office of Consumer Protection and Food Safety (BVL), Berlin, Germany), Silke Akhdar (Institute for Biochemistry; University of Veterinary Medicine Hannover, Hannover, Germany), Marie-Christin Mattauch, Anika Friese, Uwe Roesler (Institute for Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany), Axel Kramer, Paula Zwicker (Institute of Hygiene and Environmental Medicine, University Medicine, Greifswald, Germany), Christin Freitag (Landesuntersuchungsamt Rheinland-Pfalz (LUA), Koblenz, Germany) Kristina Kadlec (Dairy Herd Consulting and Research Company, (MBFG), Wunstorf, Germany), Julia Assmann, Birgit Walther (Advanced Light and Electron Microscopy (ZBS-4), Robert Koch Institute, Berlin, Germany), Christiane Cuny (Robert Koch Institute, Wernigerode Branch, Wernigerode,
Germany), Ludwig Hölzle (Universität Hohenheim, Institut für Nutztierwissenschaften, Fachgebiet, Umwelt-und Tierhygiene, Hohenheim, Germany)

Abstract:
Every laboratory test needs validation by quality controls. For biocide susceptibility testing (BST), neither quality control (QC) strains nor QC ranges applicable to these strains are currently available. As QC strains, four well-defined laboratory reference strains (Staphylococcus aureus ATCC® 6538, Enterococcus hirae ATCC® 10541, Escherichia coli ATCC® 10536 and Pseudomonas aeruginosa ATCC® 15442), which have been used previously for biocide efficacy testing, were selected. In an interlaboratory trial with eleven participating laboratories, BST QC ranges should be developed for the aforementioned four strains and the four biocides benzalkonium chloride, chlorhexidine, octenidine and polyhexanide. The performance of three different lots of tryptic soy broth was explored using the broth microdilution method and the data were subsequently evaluated using the RangeFinder software. As a result, QC ranges were defined for all reference strain-biocide combinations, except for P. aeruginosa ATCC® 15442 with the two biocides chlorhexidine and polyhexanide. The development of the latter two QC ranges was not possible, due to the limited solubility of the biocides in the test range required for P. aeruginosa ATCC® 15442. The newly developed QC ranges comprise three to five dilution steps. The establishment of QC ranges will contribute to the validation of BST in the future.