Publication Database

In vivo dynamics of oviduct-specific glycoprotein 1 (OVGP1) in dairy cows and heifers (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Neubrand, L.

Pothmann, H.

Besenfelder, U

Havlicek, V.

Aurich, C.

Gabler, C. (WE 3)

Drillich, M.

Wagner, K.

Quelle

Reproduction in domestic animals = Zuchthygiene

Bandzählung: 57

Heftzählung: S1: Special issue: Proceedings of the 24th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), 11-16 October 2021 Virtual Congress 11-16 October 2021

Seiten: 35

ISSN: 0936-6768

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1111/rda.14056

DOI: 10.1111/rda.14056

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

A better understanding of in vivo conditions in the oviduct is crucial to explain subfertility in cattle and other species. The advancement of the method of transvaginal endoscopy (TVE)-guided oviductal sampling may provide new in vivo insights into this organ. Oviduct-specific glycoprotein 1 (OVGP1) is a well-studied secretory molecule in the oviduct and has important protective functions for the developing embryo. Therefore, the aim of this study was to explore the applicability of TVE-guided sampling by investigating the dynamics of OVGP1 mRNA expression in the bovine oviduct. In total, 8 lactating dairy cows and 6 heifers were included in the study. After hormonal synchronization, animals were repeatedly sampled during the follicular (FOL) and luteal phase (CL). TVE-guided oviductal sampling was performed using a small cytobrush (2 mm diameter) that was inserted under visual control through the infundibulum into the oviduct. Total RNA was isolated from the cytobrushes and quantitative RT-PCR was performed for OVGP1. The stage of the estrous cycle was confirmed by macroscopic assessment of the ovaries during TVE and by measurement of the serum progesterone concentration (threshold for CL: ≥2 ng/ml). Differences in the mRNA abundance between FOL and CL were calculated by Wilcoxon-test. The level of significance was set at P < 0.05. The mRNA abundance of OVGP1 was greater in FOL than in CL (3.4 fold, P = 0.03). Cows showed a 2.6-fold greater OVGP1 mRNA expression compared with heifers during CL (P = 0.04). Our results confirm previous ex vivo and in vitro studies on dynamics of OVGP1 mRNA expression in the bovine oviduct. This study represents the starting point for further studies on the effects of reproductive pathologies on the complex oviductal microenvironment.