Publikationsdatenbank

Systematic analysis of different degrees of haemolysis on miRNA levels in serum and serum-derived extracellular vesicles from dogs (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Aguilera-Rojas, Matias (WE 3)

Sharbati, Soroush (WE 3)

Stein, Torsten (WE 3)

Candela Andrade, Mario

Kohn, Barbara (WE 20)

Einspanier, Ralf (WE 3)

Quelle

BMC veterinary research

Bandzählung: 18

Heftzählung: 1

Seiten: Article number: 355

ISSN: 1746-6148

Sprache

Englisch

Verweise

URL (Volltext): https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-022-03445-8

DOI: 10.1186/s12917-022-03445-8

Pubmed: 36138476

Kontakt

Klein- und Heimtierklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62422
kleintierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Circulating microRNAs (miRNAs) are described as promising non-invasive biomarkers for diagnostics and therapeutics. Human studies have shown that haemolysis occurring during blood collection or due to improper sample processing/storage significantly alters the miRNA content in plasma and serum. Nevertheless, no similar research has been performed in dogs so far. We therefore investigated the effects of different degrees of haemolysis on the levels of selected miRNAs in serum and serum-derived extracellular vesicles (EVs) from dogs, by inducing a controlled in vitro haemolysis experiment.

Results:
The abundance of miR-16, miR-92a, miR-191, miR-451 and miR-486 was significantly sensitive to haemolysis in serum and serum-derived EVs, while other selected miRNAs were not influenced by haemolysis. Furthermore, we found that the abundance of some canine miRNAs differs from data reported in the human system.

Conclusions:
Our results describe for the first time the impact of haemolysis on circulating miRNAs not only in whole serum, but also in serum-derived EVs from dogs. Hence, we provide novel data for further analyses in the discovery of canine circulating biomarkers. Our findings suggest that haemolysis should be carefully assessed to assure accuracy when investigating circulating miRNA in serum or plasma-based tests.