Publikationsdatenbank

Expression of the heterotrimeric GP2/GP3/GP4 spike of an arterivirus in mammalian cells (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Matczuk, Anna Karolina

Zhang, Minze (WE 5)

Veit, Michael (WE 5)

Ugorski, Maciej

Quelle

Viruses

Bandzählung: 14

Heftzählung: 4

Seiten: Artikel 749

ISSN: 1999-4915

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1999-4915/14/4/749

DOI: 10.3390/v14040749

Pubmed: 35458479

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Equine arteritis virus (EAV), an enveloped positive-strand RNA virus, is an important pathogen of horses and the prototype member of the Arteiviridae family. Unlike many other enveloped viruses, which possess homotrimeric spikes, the spike responsible for cellular tropism in Arteriviruses is a heterotrimer composed of 3 glycoproteins: GP2, GP3, and GP4. Together with the hydrophobic protein E they are the minor components of virus particles. We describe the expression of all 3 minor glycoproteins, each equipped with a different tag, from a multi-cassette system in mammalian BHK-21 cells. Coprecipitation studies suggest that a rather small faction of GP2, GP3, and GP4 form dimeric or trimeric complexes. GP2, GP3, and GP4 co-localize with each other and also, albeit weaker, with the E-protein. The co-localization of GP3-HA and GP2-myc was tested with markers for ER, ERGIC, and cis-Golgi. The co-localization of GP3-HA was the same regardless of whether it was expressed alone or as a complex, whereas the transport of GP2-myc to cis-Golgi was higher when this protein was expressed as a complex. The glycosylation pattern was also independent of whether the proteins were expressed alone or together. The recombinant spike might be a tool for basic research but might also be used as a subunit vaccine for horses.