Publikationsdatenbank

The proteome of the equine mucus and its compositional changes in severe equine asthma (2023)

Art

Poster

Autoren

Bartenschlager, F. (WE 12)

Dumke, F.

Weise, C.

Kuropka, B.

Gehlen, H. (WE 17)

Gruber, A. D. (WE 12)

Mundhenk, L. (WE 12)

Kongress

Annual ESVP/ECVP Congress 2022

Athen, 07. – 10.09.2022

Quelle

Journal of comparative pathology

Bandzählung: 203

Seiten: 70

ISSN: 0021-9975

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S0021997523001299

DOI: 10.1016/j.jcpa.2023.03.103

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Equine asthma is one of the most important respiratory diseases of the horse. It is characterized, among other features, by severe mucus plugging of the lower airways. The mucus is an important factor in the pathogenesis; however, its composition in healthy and affected horses is poorly characterized.

Materials and methods:
Mucus from healthy horses (n = 4) and horses with severe equine asthma (SEA; n = 6) was isolated from bronchoalveolar lavage fluid and processed for liquid chromatography-tandem mass spectrometry (LC-MS/MS). Raw data were analyzed with the MaxQuant software and peptides were identified by searching against the reference proteome of Equus caballus. The data were quantitatively analyzed using the Perseus software package. Selected proteins found to be overexpressed in the mucus of horses with SEA were further analysed by immunohistochemistry using archival tissues from healthy and SEA-affected horses (n = 4 per group).

Results:
Between 1,655 and 2,075 proteins were identified and quantified in the mucus of healthy horses, whereas only about 1,000 different proteins were detected in horses with SEA. In direct comparison to healthy horses, 111 proteins were identified in higher abundance in the mucus of horses with SEA. Proteins such as mucin 4 and polymeric immunoglobulin receptor were found more abundantly in each of the horses with SEA and were also massively localized in the airway mucus plugs, as detected by immunohistochemistry.

Conclusions:
The airway mucus protein composition is drastically changed in horses with SEA. The technique allows the identification of potential biomarkers and pathomechanisms directly from mucus.