Publication Database

Bacterial metabolites in stomach and jejunum of neonatal piglets and the influence of oral glutamine supplementation on them (2022)

Art

Poster

Autoren

Schulze Holthausen, Johannes (WE 4)

Sciascia, Quentin Leon

Schregel, Johannes

Metges, Cornelia Christiane

Zentek, Jürgen (WE 4)

Kongress

15th International Symposium on Digestive Physiology of Pigs

Rotterdam, 17. – 20.05.2022

Quelle

Animal : science proceedings

Bandzählung: 13

Heftzählung: 2

Seiten: 197

ISSN: 2772-283x

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2772283X22003569

DOI: 10.1016/j.anscip.2022.03.333

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Bacterial metabolites and the amino acid glutamine (Gln) both provide the host with energy and may improve the survival of low birth weight (LBW) piglets. This study was conducted to examine the concentration of bacterial metabolites in the stomach and jejunal digesta of LBW and normal birth weight (NBW) piglets orally supplemented with Gln.

LBW and NBW male piglets born to gilts were selected and orally supplemented with either 1 g Gln or 1.22 g alanine (Ala) per kg BW as a control group until euthanasia at 5 or 12 d. Four different groups were studied: LBW+Gln; NBW+Gln; LBW+Ala; NBW+Ala (n = 12/supplementation/age group). For analysis of short-chain fatty acids (SCFA), L-lactate and biogenic amines, stomach and distal jejunal digesta was collected, snap frozen in liquid nitrogen and stored at -80°C. Statistical analyses were performed using IBM® SPSS® Statistics (version 25, IBM, USA). Multivariate ANOVA procedure was used for testing of fixed factors and differences were checked by Tukey Test for statistical significance (P<0.05).

L-lactate in stomach digesta was higher in 12 d old piglets supplemented with Gln (P<0.05) compared to piglets supplemented with Ala. In jejunal digesta, L-lactate decreased in all groups (P<0.05) from 5 to 12 d and acetic acid and total SCFA were higher in Gln supplemented animals at 5 and 12 d (P<0.05). Butyric acid and total SCFA decreased in jejunal digesta of all groups from 5 to 12 d (P<0.05). Age revealed a significant increase in biogenic amines (P<0.05) in stomach digesta of all groups from 5 to 12 d.

Gln supplementation was associated with metabolite changes primarily observed in the stomach. This indicates that early supplementation might have an influence on metabolites in the stomach but less in the distal jejunum.