Publikationsdatenbank

Effects of dietary rye and rapeseed on microbiota and histomorphology of the colon in weaner pigs (2022)

Art

Poster

Autoren

Ellner, Carola (WE 4)

Röhe, Ilen (WE 4)

Zentek, Jürgen (WE 4)

Kongress

15th International Symposium on Digestive Physiology of Pigs

Rotterdam, 17. – 20.05.2022

Quelle

Animal : science proceedings

Bandzählung: 13

Heftzählung: 2

Seiten: 200

ISSN: 2772-283x

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2772283X2200365X

DOI: 10.1016/j.anscip.2022.03.342

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Rye and rapeseed meal (RSM) are fibre-rich alternatives to wheat and soybean meal (SBM) in pig nutrition. Dietary fibre is fermented mainly in the large intestine and may stabilize gut health by influencing intestinal microbiota and epithelial morphology. Rye has a higher content of soluble fibre than wheat and RSM more insoluble fibre than SBM. This study aimed to investigate the effect of dietary rye and RSM instead of wheat and SBM on the diversity and relative abundance of microbiota and histomorphological parameters of the gut wall in the colon ascendens of weaner pigs.

88 weaner piglets were allocated to 44 pens. Four isonitrogenous diets were fed over five weeks (n=11), based on either wheat/SBM (W-SBM), wheat/RSM (W-RSM), rye/SBM (R-SBM) or rye/RSM (R-RSM). Inclusion levels were 48% for cereals, RSM 30%, SBM 25%. Diets were analysed for insoluble (IDF) and soluble dietary fibre (SDF). After five weeks, one piglet per pen was euthanized. DNA was extracted from colonic digesta and subjected to 16S rRNA sequencing. Colonic tissue was formalin-fixed and HE-stained. Crypt depth and thickness of the lamina muscularis mucosae were measured. Statistics were carried out using 2-factorial ANOVA (cereal and protein meal as fixed factors; P<0.05).

Piglets were healthy throughout the trial. Rye-based diets had more SDF than wheat, RSM-based diets more IDF than SBM. Alpha-diversity indices were not altered. Rye did not influence relative abundance of bacterial phyla. RSM increased relative abundance of Actinobacteria (P=0.029) and Bacteroidetes (P=0.014), decreased Firmicutes (P=0.005). Histomorphological parameters of the gut wall were not affected.

This study shows that RSM caused a shift toward IDF-degrading Actinobacteria and Bacteroidetes, while rye SDF might be fermented proximal to the colon. Alterations of resident microbiota seemed not to be related to epithelial integrity.