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    Environmentally acquired Bacillus and their role in C. difficile colonization restistance (2022)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ferreira, William T.
    Hong, Huynh A.
    Adams, James R. G.
    Hess, Mateusz
    Kotowicz, Natalia K.
    Tan, Sisareuth
    Ferrari, Enrico
    Brisson, Alain
    Zentek, Jürgen (WE 4)
    Soloviev, Mikhail
    Cutting, Simon M.
    Quelle
    Biomedicines : open access journal
    Bandzählung: 10
    Heftzählung: 5
    Seiten: Artikel 930
    ISSN: 2227-9059
    Sprache
    Englisch
    Verweise
    URL (Volltext): https://www.mdpi.com/2227-9059/10/5/930
    DOI: 10.3390/biomedicines10050930
    Pubmed: 35625667
    Kontakt
    Institut für Tierernährung

    Königin-Luise-Str. 49
    14195 Berlin
    +49 30 838 52256
    tierernaehrung@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Clostridioides difficile is an environmentally acquired, anaerobic, spore-forming bacterium which ordinarily causes disease following antibiotic-mediated dysbiosis of the intestinal microbiota. Although much is understood regarding the life cycle of C. difficile, the fate of C. difficile spores upon ingestion remains unclear, and the underlying factors that predispose an individual to colonization and subsequent development of C. difficile infection (CDI) are not fully understood. Here, we show that Bacillus, a ubiquitous and environmentally acquired, spore-forming bacterium is associated with colonization resistance to C. difficile. Using animal models, we first provide evidence that animals housed under conditions that mimic reduced environmental exposure have an increased susceptibility to CDI, correlating with a loss in Bacillus. Lipopeptide micelles (~10 nm) produced by some Bacilli isolated from the gastro-intestinal (GI)-tract and shown to have potent inhibitory activity to C. difficile have recently been reported. We show here that these micelles, that we refer to as heterogenous lipopeptide lytic micelles (HELMs), act synergistically with components present in the small intestine to augment inhibitory activity against C. difficile. Finally, we show that provision of HELM-producing Bacillus to microbiota-depleted animals suppresses C. difficile colonization thereby demonstrating the significant role played by Bacillus in colonization resistance. In the wider context, our study further demonstrates the importance of environmental microbes on susceptibility to pathogen colonization.