Publikationsdatenbank

Bacteriophage-therapy against canine otitis externa and pyoderma (2022)

Art

Poster

Autoren

Dalponte, A. (WE 14)

Filor, V. (WE 14)

Kaessmeyer, S.

Fulde, M. (WE 7)

Alter, T. (WE 8)

Bäumer, W. (WE 14)

Kongress

2nd German Phage Symposium

23. – 24.05.2022

Quelle

2nd German Phage Symposium : program and abstract book : 23 – 24 May 2022 — University of Hohenheim (Hrsg.)

Hohenheim, 2022 — S. 30

Sprache

Englisch

Verweise

URL (Volltext): https://www.uni-hohenheim.de/organisation/veranstaltung/zweites-deutsches-phagen-symposium-2nd-german-phage-symposium-new-perspectives-on-phage-host-interactions-from-microbiomes-and-ecology-to-molecular-assemblies-1017130

Kontakt

Institut für Pharmakologie und Toxikologie

Koserstr. 20
14195 Berlin
+49 30 838 53221
pharmakologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Otitis externa and Pyoderma in dogs mainly caused by Pseudomonas aeruginosa and Staphylococcus pseudintermedius.
Treatment is often performed by repeated and prolonged use of antimicrobial agents, which carries an increased risk of multi-resistance. Bacteriophages could be used as an alternative therapeutic option.

Aims:
The aim of this project is to investigate the phage-skin-interaction ex vivo with infected dog skin and in vitro in a 3D canine skin model, before using it as an agent in diseased dogs. For the better permeability through the stratum corneum a nanoformulation with phages is tested.

Methods:
Bacteriophages were isolated from environmental samples and characterized. To test bacteriophages-skin-interaction ex vivo, skin from euthanized dogs was examined in Franz-type diffusion cells. A concentration of bacteriophages was added to the artificially infected skin after a period of 16 hours. Samples were collected after 8 hours of additional incubation and the colony-forming units per milliliter were determined. The skin was analyzed using immunohistochemistry and electron microscopy.
To establish the canine skin model fibroblasts, endothelial cells and keratinocytes were co-cultivated in an insert-system, to form a differentiated stratum corneum. The skin model can be used for infection experiments with bacteria and later with bacteriophages. The amount of mRNA and protein was determined and the skin was analyzed histologically.

Results:
Bacteriophages were isolated and the host range was determined. First results of the Franz-type diffusion cells showed a reduction in the detected colony forming units. The 3D skin model had differentiated keratinocytes, although adjustments in the protocol are needed for an improved formation of the epidermis.

Conclusion:
First results show that Franz-type diffusion cells can be useful for investigations of phage-skin-interactions. Further experiments are needed to investigate the interaction and the improvement of penetration with the nanoformulation.