Publication Database

Mother-offspring association:
impact of the sow’s diet on Clostridioides difficile colonisation in suckling piglets and colostrum potential against toxins in IPEC-J2 (2022)

Art

Vortrag

Autoren

Grześkowiak,, Łukasz (WE 4)

Saliu, Eva-Maria (WE 4)

Martínez‑Vallespín, Beatriz (WE 4)

Wessels, Anna (WE 4)

Vahjen, Wilfried (WE 4)

Zentek, Jürgen (WE 4)

Kongress

15th International Symposium on Digestive Physiology of Pigs

Rotterdam, 17. – 20.05.2022

Quelle

Animal : science proceedings

Bandzählung: 13

Heftzählung: 2

Seiten: 151

ISSN: 2772-283x

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2772283X22004745

DOI: 10.1016/j.anscip.2022.04.020

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Clostridioides difficile is one of the pioneer gut colonisers in pigs and it has also been documented as a major cause of enteritis outbreaks in neonatal piglets. The sow-piglet relationship is crucial for the early microbial programming in the offspring. We hypothesised that a sow’s diet rich in either high- (SBP) or low-fermentable (LNC) fibre during gestation and lactation differently influences C. difficile colonisation in suckling piglets. We also assessed whether colostrum could differentially exert protective effect against clostridial toxins on the intestinal porcine epithelial cell line IPEC-J2. Twenty sows were fed gestation and lactation diets enriched with either SBP or LNC fibre. Faeces from piglets were assessed for C. difficile and toxin B concentrations. Colostrum was collected within 10h after farrowing. IPEC-J2 were treated either with colostrum, toxins or with a combination of both. The IPEC-J2 integrity was analysed hourly (0-5h) using the trans-epithelial electrical resistance (TEER) method. Data were analysed by Kruskal-Wallis or Mann-Whitney U test (significance at p≤0.05). C. difficile concentration was lower in piglets from sows fed SBP vs. LNC along the study (p≤0.05). At day 21, lower percentage of piglets from sows fed SBP than LNC shed C. difficile (0% vs. 45%, p=0.001). Toxin B prevalence was lower in 6-day-old piglets from sows fed SBP vs. LNC (17% vs. 60%, p=0.009). Toxins decreased the integrity of IPEC-J2 in a time-dependent manner but colostrum from sows fed either SBP or LNC exerted a protective effect against toxins as indicated by TEER in IPEC-J2 cells (p≤0.05). Modulation of sow’s diet seems to be a promising tool to influence the susceptibility to colonisation by C. difficile in piglets. Independent of diet, colostrum has a potential against toxin-induced effects in IPEC-J2. The results support the hypothesis of the mother-offspring association and the early microbial programming in the porcine offspring.