Publication Database

Single-cell-sequencing in SARS-COV-2-infected hamsters sheds light on endothelial cell involvement in COVID-19 (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Nouailles, Geraldine

Wyler, Emanuel

Pennitz, Peter

Postmus, Dylan

Vladimirova, Daria (WE 5)

Kazmierski, Julia

Pott, Fabian

Dietert, Kristina (Tiermedizinisches Zentrum für Resistenzforschung)

Muelleder, Michael

Farztdinov, Vadim

Obermayer, Benedikt

Wienhold, Sandra-Maria

Andreotti, Sandro

Hoefler, Thomas (WE 5)

Sawitzki, Birgit

Drosten, Christian

Sander, Leif E.

Suttorp, Norbert

Ralser, Markus

Beule, Dieter

Gruber, Achim Dieter (WE 12)

Goffinet, Christine

Landthaler, Markus

Trimpert, Jakob (WE 5)

Witzenrath, Martin

Quelle

The European respiratory journal

Bandzählung: 58

Heftzählung: Suppl.1 : ERS International Congress 2021 abstracts

Seiten: Abstract PA2355

ISSN: 0903-1936

Sprache

Englisch

Verweise

URL (Volltext): http://erj.ersjournals.com/lookup/doi/10.1183/13993003.congress-2021.PA2355

DOI: 10.1183/13993003.congress-2021.PA2355

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Pulmonary and systemic immune responses influence disease severity in COVID-19, and could be key to therapeutic strategies.

Aims and objectives:
To investigate cellular mechanisms contributing to defense or fostering detrimental inflammatory lung injury, focusing on the clinically ill-defined involvement of endothelial cells.

Methods:
Using SARS-CoV-2-infected Syrian and Roborovski hamsters as models for moderate and severe COVID-19, respectively, a detailed and longitudinal analysis of systemic and pulmonary quantitative and qualitative immune responses was conducted. Hamster omics were corroborated with datasets from COVID-19 patients.

Results:
By integrating data from COVID-19 patients, inter-species concordance of cellular and molecular host-pathogen interactions was demonstrated. In moderate disease the earliest and strongest transcriptional response following SARS-CoV-2 infection, including pro-inflammatory genes, was exerted by monocyte-derived macrophages in lungs, while epithelial cells showed only weak gene expression program changes. Notably, endothelial cells showed no evidence for infection but reacted by strong and early expression of anti-viral as well as pro-inflammatory and T cell recruiting genes, with variations depending on cell subtypes.

Conclusions:
Analysis of Syrian hamsters infected with SARS-CoV-2 identified cell type-specific effector functions, providing detailed insights into mechanisms of COVID-19, thus informing therapeutic strategies. Extended investigations in highly susceptible Roborosvki dwarf hamsters will complement our picture of moderate and severe disease courses.