Publikationsdatenbank

Distribution and characteristics of Listeria spp. in pigs and pork production chains in Germany (2022)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Oswaldi, Verena (WE 8)

Lüth, Stefanie

Dzierzon, Janine (WE 8)

Meemken, Diana (WE 8)

Schwarz, Stefan (WE 7)

Feßler, Andrea T. (WE 7)

Félix, Benjamin

Langforth, Susann (WE 8)

Quelle

Microorganisms : open access journal

Bandzählung: 10

Heftzählung: 3

Seiten: Artikel 512

ISSN: 2076-2607

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2607/10/3/512

DOI: 10.3390/microorganisms10030512

Pubmed: 35336088

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Listeria (L.) monocytogenes is a foodborne pathogen that can cause disease, mainly in elderly, pregnant or immunocompromised persons through consumption of contaminated food, including pork products. It is widespread in the environment and can also be found in asymptomatic carrier animals, for example, in different tissues of pigs. To learn more about their nature, 16 Listeria spp. isolates found in tonsils and intestinal content of pigs and 13 isolates from the slaughterhouse environment were characterized using next-generation sequencing (NGS). A wide distribution of clonal complexes was observed in pigs, as well as in the pork production chain, suggesting multiple sources of entry. Hypervirulent clones were found in pig tonsils, showing the potential risk of pigs as source of isolates causing human disease. The presence of closely related isolates along the production chain suggests a cross-contamination in the slaughterhouse or recontamination from the same source, strengthening the importance of efficient cleaning and disinfection procedures. The phenotypical antimicrobial resistance status of L. monocytogenes isolates was examined via broth microdilution and revealed a low resistance level. Nevertheless, genotypical resistance data suggested multiple resistances in some non-pathogenic L. innocua isolates from pig samples, which might pose a risk of spreading resistances to pathogenic species.