Publikationsdatenbank

Comparison of two different measurement techniques and three sample materials for bovine haptoglobin in early postpartum Holstein Friesian dairy cows (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schmitt, Ruth (WE 18)

Staufenbiel, Rudolf (WE 18)

Pieper, Laura (WE 16)

Quelle

EC Veterinary Science

Bandzählung: 6

Heftzählung: 6

Seiten: 10 – 21

Sprache

Englisch

Verweise

URL (Volltext): https://www.ecronicon.com/ecve/ECVE-06-00396.php

Kontakt

Nutztierklinik

Königsweg 65
14163 Berlin
+49 30 838 62261
klauentierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

This research was conducted to compare two measurement techniques and three sample materials for the analysis of bovine
haptoglobin (Hp), an acute-phase protein, that can potentially be used to detect excessive postpartum inflammation in dairy cows
on both farm and individual levels. Serum Hp concentrations of 99 fresh lactating Holstein Friesian dairy cows from 10 farms in the northeastern region of Germany were assessed using both pooled and individual serum samples. A colorimetric measurement on a chemical autoanalyzer (CA) was compared to an enzyme-linked immunosorbent assay (ELISA)-test kit. Additionally, Hp levels in pooled serum, lithium heparinized (LH) plasma and ethylenediaminetetraacetic acid (EDTA) plasma samples were compared. Mean serum Hp levels varied significantly between farms. Pooled serum samples showed high correlations with the mean of individual samples. Hp (ELISA) concentrations were on average 40% lower than Hp (CA) concentrations. Dilutions interfered with the analytical precision of the ELISA. Compared to serum, Hp (CA) concentrations were 2.5 and 2.3 times higher in LH plasma and EDTA plasma, respectively, which was not observed with the ELISA. In conclusion, Hp differentiates farms regarding the inflammatory state of transition cows. Pooled samples of farms can be used for this purpose. Laboratories should specify different reference values for Hp when using colorimetric measurements on a CA or ELISA-test kits, respectively. Furthermore, dilutions need to be prepared with maximum precision. Finally, serum should be the preferred sample type for colorimetric Hp measurements.