Publikationsdatenbank

The fecal resistome of horses receiving perioperative antibiotic prophylaxis revealed antimicrobial resistance gene accumulation (2021)

Art

Vortrag

Autoren

Wolf, Silver A.

Kauter, Anne

Brombach, Julian (WE 7)

Lübke-Becker, Antina (WE 7)

Kannapin, Dania A. (WE 17)

Stöckle, Sabita D. (WE 17)

Bang, Corinna

Franzenburg, Sören

Gehlen, Heidrun (WE 17)

Wieler, Lothar H. (WE 7)

Walther, Birgit

Semmler, Torsten

Kongress

Zoonoses 2021

online, 13. – 15.10.2021

Quelle

Zoonoses 2021 - International Symposium on Zoonoses Research : joint meeting of the German Research Platform for Zoonoses and the Research Network Zoonotic Diseases : program and abstracts — Forschungsnetz zoonotische Infektionskrankheiten, German Research Platform for Zoonoses (Hrsg.)

— S. 140

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/170/attachments/90/194/Zoonoses%202021%20-%20BoA_13.10.2021-2.pdf

Kontakt

Pferdeklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62299 / 62300
pferdeklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Accumulation of antimicrobial resistance genes (ARGs) in commensal and pathogenic bacteria is an emerging global One Health issue - affecting human and veterinary medicine as well as the environment. Our recent research showed that horses receiving perioperative antibiotic prophylaxis (PAP) were increasingly colonized with zoonotic multidrug resistant (MDR) bacteria during hospital stay.

We studied horses subjected to colic surgery receiving PAP, either as a single dosage (SSG) or across 5 days (5DG). Fecal samples were collected at 3 time points (t0, t1 and t2). Sample sets of 12 horses (n = 36) were metagenome shotgun sequenced and computationally analysed.

Hospitalisation and PAP caused gut metagenome perturbations. Considering samples from hospital admission (t0), fecal resistome analysis revealed a strong ARG abundance increase at t1 (5DG = +4.6 fold change (FC), SSG = +3.6 FC) followed by a decrease at t2 (5DG = -6.8 FC, SSG = -1.3 FC). ARG accumulation was found to be statistically significant at t1 for the 5DG (p = 0.032). However, we noticed considerable inter-individual differences. ARG abundance and α-diversity were negatively correlated across all samples (r = -0.61, p = 9.9e-05).

Our preliminary results help to establish an understanding of the multifaceted effects of PAP on the gut microbiome of horses. We describe a strong increase in ARGs, especially within the 5DG, which is in line with the observed increase of MDR bacteria colonizing the equine patients.