Publikationsdatenbank

Genomic diversity of methicillin-resistant Staphylococcus aureus CC398 from diseased swine in Germany (2021)

Art

Poster

Autoren

Krüger, Henrike (WE 7)

Ji, Xing

Feßler, Andrea T. (WE 7)

Hanke, Dennis (WE 7)

Fiedler, Stefan

Jiang, Nansong

Kaspar, Heike

Wang, Yang

Wu, Congming

Schwarz, Stefan (WE 7)

Kongress

Zoonoses 2021

online, 13. – 15.10.2021

Quelle

Zoonoses 2021 - International Symposium on Zoonoses Research : joint meeting of the German Research Platform for Zoonoses and the Research Network Zoonotic Diseases : program and abstracts — Forschungsnetz zoonotische Infektionskrankheiten, German Research Platform for Zoonoses (Hrsg.)

— S. 34

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/170/attachments/90/194/Zoonoses%202021%20-%20BoA_13.10.2021-2.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objective:
Unrelated livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) CC398 of pigs from Germany were investigated for genomic diversity and evolutionary relationships to understand changes within this lineage over time.

Methodology:
Whole-genome sequences of 178 LA-MRSA-CC398 of diseased swine collected all over Germany during the period from 2007 - 2019 were investigated. The isolates were characterized by various typing methods. A minimum spanning tree was built to illustrate clonal relationships.

Results:
Sequence analysis revealed the presence of four different sequence types, which all belong to CC398. The isolates displayed 13 spa types and 23 dru types. The most common types were t011, t034 and dt11a, dt6j, respectively. One isolate harbored a SCCmec type IV element. Two isolates carried a
truncated cassette. In all remaining cases type V elements were detected. Core genome multilocus sequence typing revealed 177 allelic profiles comprising 9 clusters and 9 singletons. Phylogenetic relationships were considerably close as allelic profiles varied within a cluster in 0 to 29 and between clusters/singletons in 30 to 124 target genes of 1569 alleles included.

Conclusion:
The dominant porcine LA-MRSA clone in Germany displayed a wide genomic diversity and rather close phylogenetic relationships among isolates. Considering the zoonotic potential and the capability of spreading to other settings, deeper insight into the lineage’s characteristics is important.