Publication Database

Prevalence and characterization of Clostridioides difficile in new world camelids in Germany (2021)

Art

Poster

Autoren

Dost, Ines

Abdel-Glil, Mostafa

Schmook, Gernot

Menge, Christian

Berens, Christian

González Santamarina, Belén

Neubauer, Heinrich

Schwarz, Stefan (WE 7)

Seyboldt, Christian

Kongress

Zoonoses 2021

online, 13. – 15.10.2021

Quelle

Zoonoses 2021 - International Symposium on Zoonoses Research : joint meeting of the German Research Platform for Zoonoses and the Research Network Zoonotic Diseases : program and abstracts — Forschungsnetz zoonotische Infektionskrankheiten, German Research Platform for Zoonoses (Hrsg.)

— S. 42

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/170/attachments/90/194/Zoonoses%202021%20-%20BoA_13.10.2021-2.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Clostridioides (C.) difficile is a well-known enteric pathogen that can cause diseases in humans and animals. It is ubiquitous and a huge variety of different antibiotic resistance (AR) patterns has been detected.

New World Camelids (NWC) are becoming increasingly popular in Germany, resulting in the creation of a novel human-animal interface. However, until now, there are no data on C. difficile and its characteristics in this animal species.

Aiming at disclosing the presence and molecular characteristics of C. difficile in NWC in Germany, we collected 94 faecal samples from 43 alpaca-/llama-husbandries in 2019. Eight C. difficile isolates could be cultivated, resulting in a prevalence of 16.3% (7/43) at farm-level. All isolates carried the toxin genes A and B, one isolate was also positive for the binary toxin genes. The isolates belonged to different PCR-ribotypes, including one isolate of the potentially zoonotic ribotype RT 078. Phenotypic susceptibility against vancomycin, metronidazole and moxifloxacin was tested for via Etest; two isolates
showed resistance against moxifloxacin. Whole genome sequencing of all isolates led to the detection of different AR genes. Analysis of single nucleotide polymorphisms revealed four different clusters in accordance with the results from ribotyping and sequence typing.

This study demonstrates that NWC are capable of harbouring C. difficile and pose a potential risk for zoonotic transmission of this pathogen.