Publikationsdatenbank

Biocide susceptibility of Escherichia coli isolated from human and swine samples (2021)

Art

Poster

Autoren

Da Silva, David Attuy Vey

Dieckmann, Ralf

Makarewicz, Oliwia

Hartung, Anita

Bethe, Astrid (WE 7)

Belik, Vitaly (WE 16)

Pletz, Mathias

Al Dahouk, Sascha

Vincze, Szilvia

Kongress

Zoonoses 2021

online, 13. – 15.10.2021

Quelle

Zoonoses 2021 - International Symposium on Zoonoses Research : joint meeting of the German Research Platform for Zoonoses and the Research Network Zoonotic Diseases : program and abstracts — Forschungsnetz zoonotische Infektionskrankheiten, German Research Platform for Zoonoses (Hrsg.)

— S. 23

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/170/attachments/90/194/Zoonoses%202021%20-%20BoA_13.10.2021-2.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Biocides are essential to prevent foodborne zoonotic diseases. However, concerns have been raised that their use may contribute to the development and spread of antibiotic resistant bacteria along the food production chain and in healthcare settings. In the research project BiozAR, we investigate the susceptibility of the indicator organism E. coli isolated from livestock, food and humans to frequently used biocides and clinically relevant antibiotics to identify potential associations. Minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of six biocides were determined by broth microdilution for isolates from swine (faeces and meat, n=100 each) and humans (outpatients, n=104). Biocide susceptible isolates and isolates with reduced susceptibility were distinguished by MIC95/MBC95. Observed differences in the investigated subpopulations were statistically verified using pairwise comparison. Reduced susceptibility to biocides was found both in human and swine isolates. Overall, human isolates were less susceptible to benzalkonium chloride and glutaraldehyde (MIC and MBC), octenidine dihydrochloride (MIC) and chlorocresol (MBC) than swine isolates. However, human isolates were more susceptible to isopropanol and sodium hypochlorite. The phenotypic susceptibility testing demonstrated that the widespread use of biocides as antiseptics and disinfectants in human medicine and in the food production chain might lead to bacterial tolerance to biocides.