Publication Database

ESBL plasmid transfer by host- and country- associated E. coli within an in vitro model of the chicken caeca (2021)

Art

Vortrag

Autoren

Leng, Joy

Ritchie, Jenny

Fivian-Hughes, Amanda

van der Putten, Boas

Nguyen, Vinh Trung

Oldenkamp, Rik

Bootsma, Martin

Tiwari, Sumeet Kumar

Metamoros, Sebastien

Hoa, Ngo Thi

Berens, Christian

Alvarez, Julio

Ferrandis-Vila, Marta

Fruth, Angelika

Schwarz, Stefan (WE 7)

Bethe, Astrid (WE 7)

Mengel, Christian

Schultsz, Constance

Semmler, Torsten

La Ragione, Roberto

Kongress

Avian Infectious Diseases 2021

17.09.2021

Quelle

Sprache

Englisch

Verweise

URL (Volltext): https://microbiologysociety.org/event/society-events-and-meetings/avian-infectious-diseases-2021.html?keyword=ESBL+plasmid#tab-1

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Antimicrobial resistance (AMR) continues to be a global issue for animal and human health. ESBL plasmids confer AMR in E. coli and other bacteria. However, the impact of inoculating an in vitromodel simultaneously with multiple E. coli isolates harbouring different ESBL plasmids has not yet been explored. Here, we inoculated an in vitro model of the chicken caeca with a cocktail of 17 ESBL harbouring E. coli isolates. The isolates were associated with four different hosts (pig, cattle, humans and chickens) and isolated from four different countries (UK, Vietnam, Germany and Spain). The isolates were able to persist in the model during the 72 hour experiment, although the total CFU/ml for the isolates and number of individual isolates decreased over time. The presence of individual isolates within the model was assessed using ORFan gene multiplex PCR assays targeting genes unique to each isolate. These showed that different isolates were present in the vessels for varying lengths of time, irrespective of their host-association. No trans-conjugants were detected when 108 CFU/ml of the E. coli isolate cocktail was added to the model. However, when the vessels were inoculated with a cocktail containing 1010 CFU/ml, potential trans-conjugants were isolated in samples between taken 48 and 72 hours post addition of the cocktail. Here, we have shown that multiple ESBL-producing E. coli isolates can persist within an in vitro model of the chicken caeca and some of these appear able to transfer their ESBL plasmid to the pre-existing commensal E. coli.