Publikationsdatenbank

Imaging of bone marrow plasma cells and of their niches (2021)

Art

Buchbeitrag

Autoren

Ulbricht, Carolin

Niesner, Raluca A. (WE 2)

Hauser, Anja E.

Quelle

Bone marrow environment : methods and protocols — Marion Espéli, Karl Balabanian (Hrsg.)

1. Auflage

New York, NY: Humana Press, 2021. Methods in molecular biology ; 2308 — S. 163–176

ISBN: 978-1-0716-1424-2

Sprache

Englisch

Verweise

URL (Volltext): https://link.springer.com/protocol/10.1007/978-1-0716-1425-9_14

DOI: 10.1007/978-1-0716-1425-9_14

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Decade-long survival of plasma cells in the bone marrow has long been a puzzling matter. To understand how plasma cells are maintained and supported by survival-niches to account for lifelong antibody production demands new intravital imaging techniques that are able to follow up a single cell and their interaction with other cell types in situ. We achieved to successfully establish longitudinal imaging of the bone marrow (LIMB) that is based on an implantable endoscopic device. In this chapter, basic approaches on how to investigate plasma cell-stroma interaction and surgical implantation procedures are introduced.