Publication Database

Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Pascual-Reguant, Anna

Köhler, Ralf

Mothes, Ronja

Bauherr, Sandy

Hernández, Daniela C.

Uecker, Ralf

Holzwarth, Karolin

Kotsch, Katja

Seidl, Maximilian

Philipsen, Lars

Müller, Werner

Romagnani, Chiara

Niesner, Raluca (WE 2)

Hauser, Anja E.

Quelle

Nature Communications

Bandzählung: 12

Heftzählung: 1

Seiten: Article number: 1737

ISSN: 2041-1723

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41467-021-21994-8

DOI: 10.1038/s41467-021-21994-8

Pubmed: 33741932

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology.