Publikationsdatenbank

Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ulbricht, Carolin

Leben, Ruth

Rakhymzhan, Asylkhan

Kirchhoff, Frank

Nitschke, Lars

Radbruch, Helena

Niesner, Raluca A. (WE 2)

Hauser, Anja E.

Quelle

eLife

Bandzählung: 10

Seiten: Artikel e56020

ISSN: 2050-084x

Sprache

Englisch

Verweise

URL (Volltext): https://elifesciences.org/articles/56020

DOI: 10.7554/eLife.56020

Pubmed: 33749591

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Calcium is a universal second messenger present in all eukaryotic cells. The mobilization and storage of Ca2+ ions drives a number of signaling-related processes, stress-responses, or metabolic changes, all of which are relevant for the development of immune cells and their adaption to pathogens. Here, we introduce the Förster resonance energy transfer (FRET)-reporter mouse YellowCaB expressing the genetically encoded calcium indicator TN-XXL in B lymphocytes. Calcium-induced conformation change of TN-XXL results in FRET-donor quenching measurable by two-photon fluorescence lifetime imaging. For the first time, using our novel numerical analysis, we extract absolute cytoplasmic calcium concentrations in activated B cells during affinity maturation in vivo. We show that calcium in activated B cells is highly dynamic and that activation introduces a persistent calcium heterogeneity to the lineage. A characterization of absolute calcium concentrations present at any time within the cytosol is therefore of great value for the understanding of long-lived beneficial immune responses and detrimental autoimmunity.