Publikationsdatenbank

Seasonal host and ecological drivers may promote restricted water as a viral vector (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Dayaram, Anisha

Seeber, Peter

Courtiol, Alexandre

Soilemetzidou, Sanatana

Tsangaras, Kyriakos

Franz, Mathias

McEwen, Gayle K.

Azab, Walid (WE 5)

Kaczensky, Petra

Melzheimer, Jörg

East, Marion L.

Ganbaatar, Oyunsaikhan

Walzer, Christian

Osterrieder, Nikolaus (WE 5)

Greenwood, Alex D. (WE 5)

Quelle

The science of the total environment : an international journal for scientific research into the environment and its relationship with man

Bandzählung: 773

Seiten: Artikel 145446

ISSN: 0048-9697

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/abs/pii/S0048969721005143

DOI: 10.1016/j.scitotenv.2021.145446

Pubmed: 33588222

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In climates with seasonally limited precipitation, terrestrial animals congregate at high densities at scarce water sources. We hypothesize that viruses can exploit the recurrence of these diverse animal congregations to spread. In this study, we test the central prediction of this hypothesis - that viruses employing this transmission strategy remain stable and infectious in water. Equid herpesviruses (EHVs) were chosen as a model as they have been shown to remain stable and infectious in water for weeks under laboratory conditions. Using fecal data from wild equids from a previous study, we establish that EHVs are shed more frequently by their hosts during the dry season, increasing the probability of water source contamination with EHV. We document the presence of several strains of EHVs present in high genome copy number from the surface water and sediments of waterholes sampled across a variety of mammalian assemblages, locations, temperatures and pH. Phylogenetic analysis reveals that the different EHV strains found exhibit little divergence despite representing ancient lineages. We employed molecular approaches to show that EHVs shed remain stable in waterholes with detection decreasing with increasing temperature in sediments. Infectivity experiments using cell culture reveals that EHVs remain infectious in water derived from waterholes. The results are supportive of water as an abiotic viral vector for EHV.