Publication Database

ESBL plasmid transfer by host- and country-associated E.coli in an in vitro model of the chicken caeca (2021)

Art

Poster

Autoren

Leng, Joy

Ritchie, Jenny

Fivian-Hughes, Amanda

Van der Putten, Boas

Nguyen, Vinh Trung

Oldenkamp, Rik

Bootsma, Martin

Kumartt, Sumeet

Ngo, Thi Hoa

Berens, Christian

Alvarez, Julio

Ferrandis-Vila, Marta

Fruth, Angelika

Schwarz, Stefan (WE 7)

Bethe, Astrid (WE 7)

Menge, Christian

Schultsz, Constance

Semmler, Torsten

Ragione, Roberto La

Kongress

One Health EJP Annual Scientific Meeting 2021

Copenhagen, Denmark and online, 09. – 11.06.2021

Quelle

Abstract book of the 3rd Annual Scientific Meeting of the One Health EJP — Hosted by Statens Serum Institut and National Food Institute at the Technical University of Denmark (Hrsg.)

Copenhagen, Denmark, 2021 — S. 125

Sprache

Englisch

Verweise

URL (Volltext): https://onehealthejp.eu/wp-content/uploads/2021/06/OHEJP2021_Abstractbook.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Aim:
To assess the impact of the simultaneous inoculation multiple Escherichia coli strains harbouring different ESBL plasmids in an in vitro model of the chicken caeca.

Methods:
A continuous flow in vitro model of the chicken caeca was inoculated with a cocktail of 17 ESBL harbouring E. coli strains. These were associated with four different hosts and from four different countries. Samples from the vessels were plated onto culture media to enumerate the number of total bacteria, total E. coli, ceftiofur-resistant E. coli and the original cocktail strains (ceftiofur- and rifampicin-resistant). The fate of individual strains within the model was monitored using ORFan gene multiplex PCR assays. Finally, replica plating was used to identify any commensal E. coli that had acquired ESBL resistance genes.

Results:
The cocktail strains were able to persist during the 72-hour experiment, although the total CFU/ml and number of individual strains decreased over time. The multiplex PCR assays showed that different strains were present in the vessels for varying lengths of time. No trans-conjugants were detected when 108 CFU/ml of the E. coli isolate cocktail was added to the model. When vessels were inoculated with a 1010 CFU/ml cocktail and potential trans-conjugants were isolated in samples taken at 48 and 72 hours post inoculation.

Conclusions:
Here, we have shown that multiple ESBL-producing E. coli strains can persist within an in vitro model of the chicken caeca for 72 hours and some of these appear able to transfer their ESBL plasmid to the pre-existing commensal E. coli.