Publikationsdatenbank

The first report of mcr-1-carrying Escherichia colioriginating from animals in Serbia (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Mišić, Dušan

Kiskaroly, Ferenc

Szostak, Michael P.

Cabal, Adriana

Ruppitsch, Werner

Bernreiter-Hofer, Tanja

Milovanovic, Viktoria

Feßler, Andrea T. (WE 7)

Allerberger, Franz

Spergser, Joachim

Müller, Elke

Schwarz, Stefan (WE 7)

Braun, Sascha D.

Monecke, Stefan

Ehricht, Ralf

Korus, Maciej

Benković, Damir

Korzeniowska, Malgorzata

Loncaric, Igor

Quelle

Antibiotics : open access journal

Bandzählung: 10

Heftzählung: 9

Seiten: Artikel 1063

ISSN: 2079-6382

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2079-6382/10/9/1063

DOI: 10.3390/antibiotics10091063

Pubmed: 34572647

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003-0.00006% for chlorohexidine, of 4-6% for isopropanol and of 0.001-0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans.